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  5. Invitrogen™ Rat IL-6 ELISA Kit

Invitrogen™ Rat IL-6 ELISA Kit

Product Code. 15552057
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Quantity:
10 x 96 Tests
96 Tests
Unit Size:
1 set
Pack of 10
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Product Code. 15552057

Brand: Invitrogen™ BMS625TEN

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This item is not returnable. View return policy

Description

Includes

Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to rat IL-6
Biotin-Conjugate anti-rat IL-6 monoclonal antibody
Streptavidin-HRP
Rat IL-6 Standard lyophilized, 4ng/mL upon reconstitution
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Red-Dye
Adhesive Films

ELISA

The Rat Interleukin 6 (Rt IL-6) ELISA quantitates Rt IL-6 in rat serum or cell culture medium. The assay will exclusively recognize both natural and recombinant rat IL-6. Principle of the method The Rat IL-6 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

IL-6 is a gene that encodes a cytokine that plays a role in inflammation and the maturation of B cells. The protein produced by this gene is an endogenous pyrogen that can induce fever in people with autoimmune diseases or infections. The gene is primarily expressed at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2). Diseases associated with IL6 include Kaposi Sarcoma and Rheumatoid Arthritis, Systemic Juvenile.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number P20607
Assay Range 31.3 to 2,000 pg/mL
Assay Sensitivity 12.0 pg/mL
Conjugate HRP
Product Type ELISA
Sample Type Serum, Supernatant
Target Species Rat
For Use With (Application) ELISA
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 24498
Gene Symbol ILg6,Ifnb2
Interassay CV 10%
Intraassay CV 5
Kit Contents Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 10 x 96 Tests
Regulatory Status RUO
Sample Volume Serum 50 μL, Supernatant 50 μL
Storage Requirements 2°C to 8°C
Synonym Interferon-beta 2, B-cell stimulatory factor 2,HGF
Target IL-6
Test Time 1 hrs 20 mins
Total Assay Time 3 hrs 10 mins
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