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R&D Systems™ Recombinant Bovine Enteropeptidase/Enterokinase Protein

Product Code. 18379237
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Quantity:
10 μg
Unit Size:
10µg
This item is not returnable. View return policy

Product Code. 18379237

Brand: R&D Systems™ 4139SE010

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This item is not returnable. View return policy

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

Measured by its ability to cleave a colorimetric peptide substrate, N-carbobenzyloxy-Lys-ThioBenzyl ester (Z-Lys-SBzl), in the presence of 5,5’Dithio-bis (2-nitrobenzoic acid) (DTNB). Lu, D. et al. (1997) J. Biol. Chem. 272:31293. The specific activity is >35 nmol/min/μg, as measured under the described conditions.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number P98072
For Use With (Application) Enzyme Activity
Formulation Supplied as a 0.2 μm filtered solution in Glycerol, NaCl and HEPES.
Gene ID (Entrez) 5651
Molecular Weight (g/mol) M.W. (Observed): 34 kDa, reducing conditions 30 kDa, non-reducing conditions; M.W. (theoretical): 1.5 kDa (heavy chain C-terminal fragment), 26 kDa (light chain)
Name Enteropeptidase/Enterokinase
Quantity 10 μg
Source E. coli-derived bovine Enteropeptidase/Enterokinase protein Cys788-Lys800 (heavy chain C-terminal fragment) with an N-terminal Ala, & Ile801-His1035 (light chain)
Storage Requirements Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20°C to -70°C as supplied. 3 months, -20°C to -70°C under sterile conditions after opening.
Structural Form Disulfide-linked heterodimer
Endotoxin Concentration <1.0 EU / 1 μg of the protein by the LAL method.
Conjugate Unconjugated
Recombinant Recombinant
Purity or Quality Grade >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Protein Enteropeptidase/Enterokinase
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