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R&D Systems™ Recombinant Human IL-27 (EBI3 + p28 Heterodimer) Protein, CF
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Quantity:
10 μg
250 μg
50 μg
Unit Size:
10µg
250µg
50µg
Description
Measured in an anti-viral assay using HepG2 human hepatocellular carcinoma cells infected with encephalomyocarditis (EMC) virus. Bender H. et al. (2009) Hepatology 50:585. The ED50 for this effect is 10.0-100 ng/mL.
Specifications
Specifications
| Accession Number | Q14213.2 & Q8NEV9.2 |
| For Use With (Application) | Bioactivity |
| Formulation | Supplied as a 0.2 μm filtered solution in MOPS and NaCl with Trehalose. |
| Gene ID (Entrez) | 246778 |
| Molecular Weight (g/mol) | M.W. (Observed): 30-34 kDa (EBI3) & 24-29 kDa (p28), under reducing conditions., M.W. (Theoretical): 23 kDa (EBI3) & 25 kDa (p28) |
| Quantity | 10 μg |
| Source | Human embryonic kidney cell, HEK293-derived human IL-27 protein Human IL-27 EBI3 (Arg21-Lys229) (N-terminus) Accession # Q14213.2 Human IL-27 p28 (Phe29-Pro243) (N-terminus) Accession # Q8NEV9.2 (C-terminus) |
| Storage Requirements | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after opening. 3 months, -20°C to -70°C under sterile conditions after opening. |
| Endotoxin Concentration | <0.10 EU per 1 μg of the protein by the LAL method. |
| Gene Alias | IL27, IL-27, IL-27 p28 subunit, IL-27 subunit alpha, IL27A, IL-27-A, IL27-A, IL27p28, IL30, interleukin 27, interleukin 30, interleukin-27 subunit alpha, MGC71873, p28IL-27A |
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