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R&D Systems™ Recombinant Human Thrombopoietin/TPO GMP Protein, CF

Product Code. 30306095
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Quantity:
1 mg
50 μg
Unit Size:
1mg
50µg
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Product Code. 30306095

Brand: R&D Systems™ BTTPOGMP050

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This item is not returnable. View return policy

Description

Recombinant Protein

Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. Avanzi, G. et al. (1988) Br. J. Haematol. 69:359. The ED50 for this effect is 0.0500-0.500 ng/mL. The specific activity of Recombinant Human Thrombopoietin is >1.00 x 107 units/mg, which is calibrated against the Human Thrombopoietin reference standard (NIBSC code: 03/124).
TRUSTED_SUSTAINABILITY

Specifications

Accession Number NP_000451.1
For Use With (Application) Bioactivity
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with Trehalose.
Gene ID (Entrez) 7066
Molecular Weight (g/mol) M.W.-theoretical: 18.7 kDa, M.W.-observed: 18-19 kDa, under reducing conditions.
Quantity 50 μg
Source E. coli-derived human Thrombopoietin/Tpo protein Ser22 - Leu195 with an N-terminal Alanine Produced using non-animal reagents in an animal-free laboratory. Manufactured and tested under cGMP guidelines.
Storage Requirements Use a manual defrost freezer and avoid repeated freeze-thaw cycles. A minimum of 12 months when stored at ≤ -20 °C as supplied. Refer to lot specific COA for the Use by Date. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, ≤ -20 °C under sterile conditions after reconstitution.
Endotoxin Concentration <0.10 EU per 1 μg of the protein by the LAL method.
Gene Alias Megakaryocyte colony-stimulating factor, Megakaryocyte growth and development factor, megakaryocyte stimulating factor, MGDF, MGDFC-mpl ligand, MKCSF, MK-CSF, ML, MPL ligand, MPLLG, MPLLGMGC163194, Myeloproliferative leukemia virus oncogene ligand, THCYT1, THPO, thrombopoietin, thrombopoietin nirs variant 1, Tpo, TPOMKCSF
Conjugate Unconjugated
Reconstitution Reconstitute at 500 μg/mL in sterile water.
Purity or Quality Grade >95%, by SDS-PAGE with quantitative densitometry by Coomassie™ Blue Staining. 18732 Da ± 50 Da
Protein Thrombopoietin/THPO
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