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R&D Systems™ Recombinant Human TNF-alpha Protein
Description
Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221. The ED50 for this effect is 25-100 pg/mL.
Specifications
Specifications
| Formulation | Lyophilized from a 0.2μm filtered solution in PBS with BSA as a carrier protein. |
| Gene ID (Entrez) | 7124 |
| Molecular Weight (g/mol) | 17.5 kDa |
| Name | TNF-alpha |
| Quantity | 100 μg |
| Source | E. coli-derived human TNF-alpha protein Val77-Leu233,with and without an N-terminal Met |
| Storage Requirements | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70° C as supplied. 1 month, 2 to 8° C under sterile conditions after reconstitution. 3 months, -20 to -70° C under sterile conditions after reconstitution. |
| Gene Alias | APC1 protein, Cachectin, Cachetin, DIF, TNF, TNF, monocyte-derived, TNF-a, TNFA, TNFalpha, TNF-alphacachectin, TNFATNF, macrophage-derived, TNFG1F, TNFSF1A, TNFSF2, TNFSF2TNF superfamily, member 2, tumor necrosis factor, tumor necrosis factor (TNF superfamily, member 2), tumor necrosis factor alpha, Tumor necrosis factor ligand superfamily member 2, tumor necrosis factor-alpha |
| Conjugate | Unconjugated |
| Recombinant | Recombinant |
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