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R&D Systems™ Recombinant TEV Protease Protein

Product Code. 18107440
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Quantity:
200 μg
Unit Size:
200µg
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Product Code. 18107440

Brand: R&D Systems™ 4469TP200

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This item is not returnable. View return policy

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Enzyme Activity

Measured by its ability to cleave a fusion protein containing the recognition sequence Glu-Asn-Leu-Tyr-Phe-Gln, with the cleavage point after Gln. TEV Protease cleaves =50% of the control substrate, as measured under the described conditions. It is recommended that the cleavage for each fusion protein be optimized by varying the amount of Recombinant Viral TEV Protease, reaction time, or incubation temperature.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number NP_062908
For Use With (Application) Enzyme Activity
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl, EDTA, DTT and Glycerol.
Gene ID (Entrez) 1502321
Molecular Weight (g/mol) M.W. (Observed): 25-30 kDa, reducing conditions; M.W. (theoretical): 28.5 kDa
Name Sleeping Beauty Transposase
Quantity 200 μg
Source E. coli-derived viral TEV Protease protein Ala TEV Protease Glu2039-Gln2279 (Ser2256Asn) (N-terminus) Accession # NP_062908 Leu 6-His tag (C-terminus)
Storage Requirements Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20°C to -70°C as supplied. 3 months, -20°C to -70°C under sterile conditions after opening.
Endotoxin Concentration <1.0 EU / 1 μg of the protein by the LAL method.
Gene Alias NIa, P1 Protease, Tobacco Etch Virus Protease
Conjugate Unconjugated
Recombinant Recombinant
Purity or Quality Grade >80%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
Protein TEV Protease
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