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  5. Invitrogen™ Human SDF-1 alpha/CXCL12A ELISA Kit

Invitrogen™ Human SDF-1 alpha/CXCL12A ELISA Kit

Artikelnummer. 17155409
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Quantity:
5 x 96 Tests
96 Tests
Pakningsstørrelse:
1 set
Each
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Artikelnummer. 17155409

Brand: Invitrogen™ EHCXCL12AX5

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ELISA

The Human SDF-1alpha/CXCL12A (SDF-1?) ELISA quantitates Hu SDF-1? in human serum,plasma,or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu SDF-1?. Principle of the method The Human SDF-1? solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples,standards,or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody,a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity,specificity,precision,and lot-to-lot consistency. See manual for more information on validation.

CXCL12 is a stromal cell-derived alpha chemokine member of the intercrine family. This gene product and its receptor CXCR4 can activate lymphocytes and have been implicated in the metastasis of some cancers such as breast cancer. Mutations in this gene are associated with resistance to human immunodeficiency virus type 1 infections. Multiple transcript variants encoding different isoforms have been found for this gene.
TRUSTED_SUSTAINABILITY

Tekniske data

Accession Number P48061
Assay Range 93.75 to 6000 pg/mL
Assay Sensitivity 80 pg/mL
Calibration Range 80 pg/mL to 6000 pg/mL
Description SDF-1 A (CXCL12A) ELISA Kit, 96 Assays, 1 Kit, Human Species, HRP Conjugate, Colorimetric Detection Method, Enzyme-Linked Immunosorbent Assay Designed for the Quantitation and Detection of SDF-1 A (CXCL12A) in Samples Using a Microplate Reader
Detectable Analytes SDF-1 A (CXCL12A), Human SDF-1 A
Assay Solid-phase sandwich ELISA
Conjugate HRP
Detection Method Colorimetric
Product Type ELISA
Protein Family Chemokine & Receptors
Sample Type Plasma, Serum, Supernatant
Specificity This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Sufficient For 5 x 96 assays
Target Species Human
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 6387
Gene Symbol IRH,PBSF,SCYB12,SDF1,TLSF,TPAR1
Host Species Human
Interassay CV <12%
Intraassay CV <10
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 5 x 96 Tests
Regulatory Status RUO
Research Discipline Signal Transduction
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
Storage Requirements 2°C to 8°C
Synonym IRH, PBSF, SCYB12, SDF1, TLSF, TPAR1
Target CXCL12 alpha
Test Time 1 hrs 20 mins
Total Assay Time 4 hrs 45 mins
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