MCAM (CD146) Recombinant Human Protein, hIgG1-Fc Tag, Invitrogen™ Sino Biological™
This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Gly 559) of human MCAM precursor (NP_006491.2) fused to the Fc region of human IgG1 at the C-terminus
Brand: Invitrogen 10115H02H250
Additional Details : Weight : 0.01000kg
DescriptionMelanoma cell adhesion molecule (MCAM/CD146), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in cell biology research applications. It can also be used as a protein standard.
- Activity: Measured by the ability of the immobilized protein to support the adhesion of the HUVEC human umbilical vein endothelial cell line; when 3 x 104 cells/well are added to human CD146 coated plates (0.8μg/mL, 100μL/well), approximately >30 % will adhere after one hour at 37°C
- Formulation: Lyophilized in PBS, pH 7.4, 5% Mannitol, 5% Trehalose, and 0.02% Tween 80
- Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible
Shipping Condition: Room Temperature. This protein is manufactured by Sino Biological Inc.
|Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories|
|≥90% by SDS-PAGE|
|Store at -20° to -70°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.|
|Melanoma cell adhesion molecule|
|IgG1 Fc Tag|
|5 x 50μg|
|MCAM, CD146, MUC18|
For Research Use Only. Not for use in diagnostic procedures.