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Sp2/0-Ag14 (mouse hybridoma) Whole cell lysate, Non-denatured; Abnova

Produktkod. 16059645
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Quantity:
200 μg
Förpackningsstorlek:
200µg
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Produktkod. 16059645 Leverantör Abnova Leverantörsnummer L066V2.200ug

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Beskrivning

Western Blotting, Immunoprecipitation

Specifikationer

For Use With (Application) Immunoprecipitation, Western Blot
Tissue B Lymphocyte
Description Whole cell lysate (non-denatured)
Lysis Buffer Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Preparation Method Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay).
Quality Control Testing 12.5% SDS-PAGE Stained with Coomassie Blue.
Quantity 200 μg
Storage Buffer In modified RIPA Lysis Buffer.
Host Species Mouse
Content And Storage Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Produkttitel
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