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IBA Lifesciences StarGate pESG-IBA Vectors for Mammalian Expression
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Vector:
pESG-IBA 102
pESG-IBA 103
pESG-IBA 104
pESG-IBA 105
pESG-IBA 123
pESG-IBA 142
pESG-IBA 143
pESG-IBA 145
pESG-IBA 162
pESG-IBA 164
pESG-IBA 167
pESG-IBA 168
pESG-IBA 23
pESG-IBA 25
pESG-IBA 3
pESG-IBA 33
pESG-IBA 35
pESG-IBA 43
pESG-IBA 45
pESG-IBA 5
pESG-IBA 62
pESG-IBA 63
pESG-IBA 64
pESG-IBA 65
pESG-IBA wt1
pESG-IBA wt2
Förpackningsstorlek:
5µg
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Produktkod. 15693778

Brand: IBA Lifesciences 54423001

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Beskrivning

Designed for high-level constitutive expression of recombinant proteins in a wide range of Mammalian host cells through the CMV promoter, with replication in cell lines that are latently infected with SV40 large T antigen.

pESG-IBA mammalian expression vectors

pESG-IBA is a small expression vector recommended for transient expression of proteins in cell lines providing SV40 large T-antigen for extrachromosomal replication with SV40 ori.

  • NeoR for generation of stable cells lines.
  • Cytomegalovirus immediate-early (CMV) promoter: high level constitutive expression
  • SV40 ori: origin of replication for extrachromosomal replication driven by SV40 large T-antigen (as provided by HEK293T or COS-1, -3, -7 cells)
  • ColE1 + Amp: for propagation in E. coli
  • NeoR: neomycin resistance gene to generate stable cell lines
  • BM40: secretion signal to transfer the expressed protein into the medium (not for all vectors available)

Please note that cloning into pESG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pESG-IBA vectors with Esp3I, another option via a so-called Entry-Vector is possible.since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pESG-IBA vectors with Esp3I, another option via a so-called Entry-Vector is possible.

Specifikationer

Format Liquid
For Use With (Equipment) StarGate™ System, Mammalian Host Cells
Inducing Agent SV40 large T antigen (e.g. COS1 or COS7)
Includes Neomycin resistance gene
pH 8
Promoter Human CMV Immediate-early
Product Type Acceptor Vector for mammalian expression
Storage Buffer 20 μL TE buffer: 10 mm Tris-HCl, 1 mm EDTA / 5 μg
Content And Storage 2°C to 8°C for frequent usage, -20°C for long-term storage
Protein Tag Strep-tag for C-terminal and GST-tag for N-terminal
Concentration 250 ng/μL
Quantity 5 μg
Vector pESG-IBA 23
Color Clear
For Use With (Application) Cloning Genes of Interest for Use in Functional Expression, Purification, Detection, Immobilization or Separation of Recombinant Proteins
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