IBA Solutions For Life Science StarGate™ pESG-IBA Acceptor Vectors for Mammalian Expression

Description

pESG-IGA mammalian expression vectors

pESG-IBA is a small expression vector recommended for transient expression of proteins in cell lines providing SV40 large T-antigen for extrachromosomal replication with SV40 ori. NeoR for generation of stable cells lines.

• Cytomegalovirus immediate-early (CMV) promoter: high level constitutive expression
• SV40 ori: origin of replication for extrachromosomal replication driven by SV40 large T-antigen (as provided by HEK293T or COS-1, -3, -7 cells)
• ColE1 + Amp: for propagation in E. coli
• NeoR: neomycin resistance gene to generate stable cell lines
• BM40: secretion signal to transfer the expressed protein into the medium (not for all vectors available)

StarGate™ System

IBA developed StarGate to provide a simple, fast and highly efficient cloning process for genes of interest (GOI) for use in functional expression, purification, detection, immobilization or separation of recombinant proteins:

• Minimal extra modification of the gene due to short combinatorial sites
• Inherent high-level cloning efficiency due to a directed reaction (no equilibrium)
• GOIs are cloned to obtain a Donor Vector; 2 or more genes can be combined using Fusion Cloning
• Genes are then transferred into Acceptor Vectors that provide optimal genetic surroundings
• The final expression vector, the Destination Vector, is then placed into the respective host