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IBA Solutions For Life Science StarGate™ pLSG-IBA Acceptor Vectors for Baculo
Transfer vectors that allow for high-level expression in insect cells by introducing the gene of interest into the Polyhedrin gene locus of AcMNPV DNA by homologous recombination.
Brand: IBA Solutions For Life Science 5-4803-001
Additional Details : Poids : 0.00010kg
Description
pLSG-IGA Bacula virus (insect cells) expression vectors
The pLSG-IBAwt1 vector carries the Polyhedrin promoter for high-level expression in insect cells. Co-transfection with BacPAK6 linearized AcMNPV DNA (Clontech) or circular flashBAC modified AcMNPV DNA (Oxford Expression Technologies) allows the generation of recombinant baculovirus at very high efficiency through reconstitution of an essential gene (ORF 1629) and elimination of wild type virus to great extent.
- Cloning and expression of recombinant proteins in insect cells
- Polyhedrin promoter drives high-level expression of the insert
- Ampicillin resistance and ColE1 origin of replication (pUC) support propagation in E. coli
StarGate™ System
IBA developed StarGate to provide a simple, fast and highly efficient cloning process for genes of interest (GOI) for use in functional expression, purification, detection, immobilization or separation of recombinant proteins:
- Minimal extra modification of the gene due to short combinatorial sites
- Inherent high-level cloning efficiency due to a directed reaction (no equilibrium)
- GOIs are cloned to obtain a Donor Vector; 2 or more genes can be combined using Fusion Cloning
- Genes are then transferred into Acceptor Vectors that provide optimal genetic surroundings
- The final expression vector, the Destination Vector, is then placed into the respective host
Specifications
| 250 ng/μL | |
| Clear | |
| StarGate™ system, insect host cells | |
| BacPAK6 linearized AcMNPV DNA (Clontech) or circular flashBAC modified AcMNPV DNA (Oxford Expression Technologies) | |
| 8 | |
| Acceptor Vector for bacula (insect cell) expression | |
| 2-8°C for frequent usage, -20°C for long-term storage | |
| Strep-tag™ for C-terminal |
| Cloning genes of interest for use in functional expression, purification, detection, immobilization or separation of recombinant proteins | |
| Liquid | |
| pLSG-IBA 3 | |
| pUC ori, Ampicillin resistance | |
| Polyhedrin | |
| 5 μg | |
| 20μL TE buffer: 10mM Tris-HCl, 1mM EDTA per 5μg |