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IBA Lifesciences StarGate pPSG-IBA Acceptor Vectors for E. coli Expression

Achieves high-level expression in E. coli by the T7 promoter by providing specified genetic surroundings for optimal expression and purification of non-toxic proteins.

220.00€

Specifications

Format Liquid
For Use With (Equipment) StarGate™ System, E. Coli BL21(DE3)
Inducing Agent E. coli host with T7 RNA polymerase gene (e.g. BL21(DE3))
pH 8
Promoter T7
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Products 22
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Product Code Brand Description Protein Tag Price Quantity & Availability  
15633758
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IBA Lifesciences
5-4200-001
pPSG-IBA wt1 Untagged This item has been discontinued and is no longer available. View the product for possible alternatives or contact our Technical Support team on 056 260 260 for assistance.
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15643758
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5-4203-001
pPSG-IBA 3 Strep-tag for C-terminal This item has been discontinued and is no longer available. View the product for possible alternatives or contact our Technical Support team on 056 260 260 for assistance.
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15653758
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5-4205-001
pPSG-IBA 5 Strep-tag for N-terminal This item has been discontinued and is no longer available. View the product for possible alternatives or contact our Technical Support team on 056 260 260 for assistance.
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15663758
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5-4223-001
pPSG-IBA 23 Strep-tag for C-terminal and GST-tag for N-terminal This item has been discontinued and is no longer available. View the product for possible alternatives or contact our Technical Support team on 056 260 260 for assistance.
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15673758
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5-4225-001
pPSG-IBA 25 GST Tag for N-terminal This item has been discontinued and is no longer available. View the product for possible alternatives or contact our Technical Support team on 056 260 260 for assistance.
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15683758
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5-4233-001
pPSG-IBA 33 His Tag for C-terminal This item has been discontinued and is no longer available. View the product for possible alternatives or contact our Technical Support team on 056 260 260 for assistance.
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15693758
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5-4235-001
pPSG-IBA 35 His Tag (6x) for N-terminal This item has been discontinued and is no longer available. View the product for possible alternatives or contact our Technical Support team on 056 260 260 for assistance.
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15603768
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5-4243-001
pPSG-IBA 43 Strep-tag for C-terminal and His Tag (6x) for N-terminal This item has been discontinued and is no longer available. View the product for possible alternatives or contact our Technical Support team on 056 260 260 for assistance.
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15613768
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5-4245-001
pPSG-IBA 45 Strep-tag for N-terminal and His Tag (6x) for C-terminal This item has been discontinued and is no longer available. View the product for possible alternatives or contact our Technical Support team on 056 260 260 for assistance.
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15623768
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5-4262-001
pPSG-IBA 62 Strep-tag for C-terminal and FLAG-tag for N-terminal This item has been discontinued and is no longer available. View the product for possible alternatives or contact our Technical Support team on 056 260 260 for assistance.
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15633768
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5-4263-001
pPSG-IBA 63 FLAG-tag for C-terminal This item has been discontinued and is no longer available. View the product for possible alternatives or contact our Technical Support team on 056 260 260 for assistance.
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15643768
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IBA Lifesciences
5-4264-001
pPSG-IBA 64 Strep-tag for N-terminal and FLAG-tag for C-terminal This item has been discontinued and is no longer available. View the product for possible alternatives or contact our Technical Support team on 056 260 260 for assistance.
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15653768
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5-4265-001
pPSG-IBA 65 FLAG-tag for N-terminal This item has been discontinued and is no longer available. View the product for possible alternatives or contact our Technical Support team on 056 260 260 for assistance.
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15663768
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IBA Lifesciences
5-4303-001
pPSG-IBA 103 Twin-Strep-tag for C-terminal This item has been discontinued and is no longer available. View the product for possible alternatives or contact our Technical Support team on 056 260 260 for assistance.
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15673768
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IBA Lifesciences
5-4305-001
pPSG-IBA 105 Twin-Strep-tag for N-terminal This item has been discontinued and is no longer available. View the product for possible alternatives or contact our Technical Support team on 056 260 260 for assistance.
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15683768
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IBA Lifesciences
5-4323-001
pPSG-IBA 123 Twin-Strep-tag for C-terminal and GST-tag for N-terminal This item has been discontinued and is no longer available. View the product for possible alternatives or contact our Technical Support team on 056 260 260 for assistance.
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15693768
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IBA Lifesciences
5-4343-001
pPSG-IBA 143 Twin-Strep-tag for C-terminal and His Tag (6x) for N-terminal This item has been discontinued and is no longer available. View the product for possible alternatives or contact our Technical Support team on 056 260 260 for assistance.
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15603778
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5-4345-001
pPSG-IBA 145 Twin-Strep-tag for N-terminal and His Tag (6x) for C-terminal This item has been discontinued and is no longer available. View the product for possible alternatives or contact our Technical Support team on 056 260 260 for assistance.
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15613778
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5-4362-001
pPSG-IBA 162 Twin-Strep-tag for C-terminal and FLAG-tag for N-terminal This item has been discontinued and is no longer available. View the product for possible alternatives or contact our Technical Support team on 056 260 260 for assistance.
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15623778
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IBA Lifesciences
5-4364-001
pPSG-IBA 164 Twin-Strep-tag for N-terminal and FLAG-tag for C-terminal This item has been discontinued and is no longer available. View the product for possible alternatives or contact our Technical Support team on 056 260 260 for assistance.
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15633778
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IBA Lifesciences
5-4367-001
pPSG-IBA 167 Twin-Strep-tag/FLAG-tag for N-terminal This item has been discontinued and is no longer available. View the product for possible alternatives or contact our Technical Support team on 056 260 260 for assistance.
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15643778
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IBA Lifesciences
5-4368-001
pPSG-IBA 168 Twin-Strep-tag/FLAG-tag for C-terminal This item has been discontinued and is no longer available. View the product for possible alternatives or contact our Technical Support team on 056 260 260 for assistance.
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Description

Description

pPSG-IGA E. coli expression vectors:

pPSG-IBA vectors use the T7 promoter and T7 RNA polymerase for high-level transcription of the gene of interest.

  • As the T7 promoter is stronger than the tet promoter, pPSG-IBA vectors are recommended in cases where expression with the tet promoter does not lead to significant yields of the recombinant protein.

    • High-level expression in E. coli by bacteriophage T7 promoter
    • High-level transcription by T7 RNA polymerase in BL21 strains
    • High-level expression of non-toxic proteins
    • Induction by IPTG
    • Suitable for in vitro transcription/translation

    StarGate System

    IBA developed StarGate to provide a simple, fast and highly efficient cloning process for genes of interest (GOI) for use in functional expression, purification, detection, immobilization or separation of recombinant proteins:

    • Minimal extra modification of the gene due to short combinatorial sites
    • Inherent high-level cloning efficiency due to a directed reaction (no equilibrium)
    • GOIs are cloned to obtain a Donor Vector; 2 or more genes can be combined using Fusion Cloning
    • Genes are then transferred into Acceptor Vectors that provide optimal genetic surroundings
    • The final expression vector, the Destination Vector, is then placed into the respective host
  • Specifications

    Specifications

    Liquid
    E. coli host with T7 RNA polymerase gene (e.g. BL21(DE3))
    T7
    20 μL TE buffer: 10 mm Tris-HCl, 1 mm EDTA / 5 μg
    250 ng/μL
    Total length: 3469
    Cloning genes of interest for use in functional expression, purification, detection, immobilization or separation of recombinant proteins
    StarGate™ System, E. Coli BL21(DE3)
    8
    Acceptor Vector for E. coli expression
    2°C to 8°C for frequent usage, -20°C for long-term storage
    5 μg
    Clear
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