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IBA Solutions For Life Science StarGate™ pPSG-IBA Acceptor Vectors for E. coli Expression

Achieves high-level expression in E. coli by the T7 promoter by providing specified genetic surroundings for optimal expression and purification of non-toxic proteins.

Brand:  IBA Solutions For Life Science 5-4223-001

Additional Details : Poids : 0.00010kg

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Product Code. 15663758

  • 213.00€ / 5µg


Expédition estimée
30-09-2020
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Description

Description

pPSG-IGA E. coli expression vectors:

pPSG-IBA vectors use the T7 promoter and T7 RNA polymerase for high-level transcription of the gene of interest. As the T7 promoter is stronger than the tet promoter, pPSG-IBA vectors are recommended in cases where expression with the tet promoter does not lead to significant yields of the recombinant protein.

  • High-level expression in E. coli by bacteriophage T7 promoter
  • High-level transcription by T7 RNA polymerase in BL21 strains
  • High-level expression of non-toxic proteins
  • Induction by IPTG
  • Suitable for in vitro transcription/translation

StarGate™ System

IBA developed StarGate to provide a simple, fast and highly efficient cloning process for genes of interest (GOI) for use in functional expression, purification, detection, immobilization or separation of recombinant proteins:

  • Minimal extra modification of the gene due to short combinatorial sites
  • Inherent high-level cloning efficiency due to a directed reaction (no equilibrium)
  • GOIs are cloned to obtain a Donor Vector; 2 or more genes can be combined using Fusion Cloning
  • Genes are then transferred into Acceptor Vectors that provide optimal genetic surroundings
  • The final expression vector, the Destination Vector, is then placed into the respective host
Specifications

Specifications

250 ng/μL
Liquid
pPSG-IBA 23
Cloning genes of interest for use in functional expression, purification, detection, immobilization or separation of recombinant proteins
8
Acceptor Vector for E. coli expression
Total length: 3469
20μL TE buffer: 10mM Tris-HCl, 1mM EDTA per 5μg
Clear
StarGate™ system, E. coli BL21(DE3)
E. coli host with T7 RNA polymerase gene (e.g. BL21(DE3) )
Ampicillin Resistance cassette
T7
5 μg
2-8°C for frequent usage, -20°C for long-term storage
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