IBA Solutions For Life Science StarGate™ pYSG-IBA Acceptor Vectors for Yeast Expression

Description

pYSG-IGA yeast expression vectors

The pYSG-IBA3 vector allows the high-level expression of proteins in yeast. The vector carries the copper inducible promoter (CUP1) for controlled high-level expression. The expressed recombinant protein will be localized in the cytoplasm.

• High-level expression in yeast with high copy number
• Favors correct protein folding and the production of soluble proteins - inclusion bodies rarely form
• Tightly regulated expression due to Cu⁺⁺-inducible CUP1 promoter
• Ampicillin resistance gene and markers Leu2-d and URA3 in every vector
• Leu2-d auxotrophy marker for selection to increase plasmid copy number during expression
• URA3 auxotrophy marker for selection after transformation

StarGate™ System

IBA developed StarGate to provide a simple, fast and highly efficient cloning process for genes of interest (GOI) for use in functional expression, purification, detection, immobilization or separation of recombinant proteins:

• Minimal extra modification of the gene due to short combinatorial sites
• Inherent high-level cloning efficiency due to a directed reaction (no equilibrium)
• GOIs are cloned to obtain a Donor Vector; 2 or more genes can be combined using Fusion Cloning
• Genes are then transferred into Acceptor Vectors that provide optimal genetic surroundings
• The final expression vector, the Destination Vector, is then placed into the respective host