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STEMCELL Technologies StemSpan™ T Cell Generation Kit

Product Code. 17179961
Encompass
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Quantity:
1 Kit
Unit Size:
1 set
This item is not returnable. View return policy

Product Code. 17179961

Brand: STEMCELL Technologies 09940

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This item is not returnable. View return policy

Description

For expansion and differentiation of human CD34+ hematopoietic progenitor cells to T cells

  • Use this two-step protocol to reproducibly expand thousands of CD4+CD8+ double-positive (DP) T cells in cultures initiated with CD34+ human cord blood (CB) cells
  • In step one, CD34+ CB cells are cultured for 14 days in medium containing expansion supplement to promote their proliferation and differentiation into CD7+CD5+ pro-T cells
  • In step two, pro-T cells generated during those first 14 days are cultured for another 4 weeks in medium containing maturation supplement to promote their maturation into DP cells
  • This product is serum- and feeder-free, which helps eliminate variation introduced by serum and stromal cell lines
  • An optional protocol is available to further mature DP cells to CD8 SP T cells
  • This extended protocol uses complete T cell progenitor maturation medium made with reagents included in the StemSpan™ T Cell Generation Kit and which must be combined with ImmunoCult™ CD3/CD28/CD2 T Cell Activator (Catalog #10970 or #10971) and IL-15 (Catalog #78031)

Specifications

Cell Type Hematopoietic Stem and Progenitor Cells, T Cells
For Use With (Application) Cell Culture; Differentiation; Expansion
Quantity 1 Kit
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