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Cytiva StrepTactin™ Sepharose™ High Performance Medium
Mild purification conditions preserve antigenicity and function. Cytiva StrepTactin Sepharose High Performance media is used for robust, high resolution purification of Strep II-tagged proteins.
Brand: Cytiva 28-9356-00
Additional Details : CAS : 79-06-1 Weight : 0.11400kg
Description
- Medium with high binding capacity yields high resolution, sharp concentrated peaks, and reproducible purifications
- Physiological conditions and mild elution preserves the activity of the target protein
- Compatible with a wide range of reducing agents, detergents, denaturants, and other additives
- Easily scaled up, and easily regenerated with 0.5M NaOH
- Convenient use with a peristaltic pump or chromatography system
- Matrix: rigid, highly cross-linked 6% agarose
- Ave. particle size: 34μm
- Dynamic binding capacity (protein dependent): approx. 6mg Strep(II)-tagged protein/mL medium
- Compatibility: stable in all commonly used buffers, 0.5M NaOH (regeneration and cleaning), reducing agents, denaturants, and detergents
- pH stability: >7
Specifications
| StrepTactin Sepharose HP media | |
| <300 cm/hr. | |
| 7 | |
| 6% Highly Cross-linked Agarose | |
| Purification of Strep (II) -tagged recombinant Proteins to high purities in concentrated forms and small volumes by using with perstaltic pump or chromato graphy system |
| 50mL | |
| StrepTactin (∼5mg StrepTactin/mL medium) | |
| 34μm | |
| 2° to 8°C | |
| Strep-Tactin Sepharose |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- StrepTactin Sepharose High Performance, 50 ml
Signal Word
- Warning
Hazard Category
- Flammable liquid Category 3
Hazard Statement
- H226-Flammable liquid and vapour.
Precautionary Statement
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
- P241-Use explosion-proof electrical/ventilating/lighting/and all material-handling/ equipment.
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P235-Keep cool.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
- MIXTURE LIST-Contains : ethanol