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  7. Invitrogen™ SuperScript™ IV Template Switching RT Master Mix

Invitrogen™ SuperScript™ IV Template Switching RT Master Mix

Product Code. 18074100
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No. of Reactions:
48 Reactions
5 x 96 Reactions
96 Reactions
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48 reactions
96 reactions
Pack of 5
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Product Code. No. of Reactions unitSize
18074100 96 Reactions 96 reactions
18004080 48 Reactions 48 reactions
30055899 5 x 96 Reactions Pack of 5
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Product Code. 18074100 Supplier Invitrogen™ Supplier No. A65424

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789.60 EUR valid until 2026-02-06
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Description

Invitrogen SuperScript IV Template-Switching RT Master Mix is designed for high template switching efficiency in cDNA synthesis reactions.

Invitrogen SuperScript IV Template-Switching RT Master Mix is designed for high template switching efficiency in cDNA synthesis reactions. It contains SuperScript IV Reverse Transcriptase, RNase Inhibitor, and all other reverse transcription reaction components in a convenient premixed format. Only RNA template, RT primer, and template switching oligo (TSO) of choice need to be added.

Features of SuperScript IV Template-Switching RT Master Mix include:
• Formulated for high quality full-length cDNA synthesis
• Includes proprietary enhancer to maximize template switching efficiency
• Exceptional sensitivity for easy detection of low abundance targets (as low as single cell or 2 pg total RNA)
• Cell lysis buffer included for direct cDNA synthesis without RNA purification

Procedure overview
To maximize template switching efficiency, SuperScript IV Template-Switching RT Master Mix contains proprietary template switching enhancer and leverages the terminal deoxynucleotidyl transferase (TdT) activity of SuperScript IV Reverse Transcriptase. When the reverse transcriptase reaches the 5’ end of the RNA template, TdT activity adds 1–3 extra nucleotides to the cDNA end, enabling binding of the template switching oligo and incorporation of known sequence at the 3’ end of the resulting cDNA. This cDNA can be used for amplification by PCR, gene expression analysis by real-time PCR, or as a template for 5’RACE, RNA-seq, or second strand cDNA synthesis.This master mix is compatible with a wide input range of total RNA (from 2 pg to 4 µg) and is supplied with lysis buffer to enable direct cDNA synthesis from mammalian cells (1–1,000 cells per sample).

Related products
SuperScript IV Template-Switching RT Master Mix is compatible with various RT primers and self-designed template switching oligos. We offer Template Switching Oligo (Cat. No. A65427) and Capturing Oligo (dT) Primer (A65429), both containing the same adaptor sequence complementary to Preamplification Primer (A65428). Alternatively, a full set of reagents for template switching cDNA synthesis and preamplification is available in the SuperScript IV Single Cell/Low-Input cDNA PreAmp Kit (11752048).

TRUSTED_SUSTAINABILITY

Specifications

Concentration 4X
Content And Storage • 4X SuperScript IV cDNA Synthesis Master Mix (480 μL)
• 10X Lysis Buffer (96 μL)
• Nuclease-free water (1.25 mL)

Store at -15 to -25°C.
Format Master Mix
GC-Rich PCR Performance High
Reaction Speed 30 min.
Technique Template Switching Reverse Transcription
Quantity 96 reactions
Final Product Type First-Strand cDNA
No. of Reactions 96 Reactions
Reagent Type Reverse Transcription
Starting Material Cells or RNA
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For Research Use Only. Not for use in diagnostic procedures.

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