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SW-13 (human small cell carcinoma of the adrenal cortex) Whole cell lysate, Denatured; Abnova

Product Code. 16019505
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Quantity:
200 μg
Unit Size:
200µg
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Product Code. 16019505

Brand: Abnova L005V1.200ug

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This item is not returnable. View return policy

Description

Western Blotting

Specifications

For Use With (Application) Western Blot
Tissue Adrenal Gland (Cortex)
Description Whole cell lysate (denatured)
Lysis Buffer Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Preparation Method Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The lysate was mixed in 5X Sample Buffer to become final 1X Sample Buffer as Storage Buffer. The lysate was heated at 95°C for 5 min, and cooled rapidly.
Quality Control Testing 12.5% SDS-PAGE Stained with Coomassie Blue.
Quantity 200 μg
Storage Buffer In ready-to-use 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue).
Host Species Human
Concentration 3 mg/mL
Content And Storage Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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