T-bet, PerCP-Cyanine5.5, clone: eBio4B10 (4B10), eBioscience™
Mouse Monoclonal Antibody
Marque: Affymetrix eBioscience 45-5825-82
Informations supplémentaires : Poids : 0.25000kg
DescriptionDescription: The eBio4B10 monoclonal antibody reacts with mouse and human T-bet. T-bet is a Th1-specific T-box transcription factor critical to the development of the Th1 CD4+ T cell lineage. This is known based on the observations that T-Bet deficient mice have impaired Th1 cell development, and that ectopic expression of T-Bet results in development skewed to the Th1 lineage. T-Bet expression is induced by the Th1 cytokine IFN gamma, and T-Bet also regulates the expression of IFN gamma, likely, at least in part, through the modification of DNA accessibility and histone remodeling. In addition to IFN gamma, T-Bet is also known to regulate the expression of IL-12R beta and IL-2. Moreover, T-Bet plays a role in class-switch recombination in B-cells.Applications Reported: This eBio4B10 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This eBio4B10 antibody has been tested by intracellular staining and flow cytometric analysis of normal human peripheral blood cells. This antibody can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.For intracellular staining with the eBio4B10 monoclonal antibody, it is recommended to use the Foxp3/Transcription Factor Staining Buffer Set (cat. 00-5523) and protocol. Please refer to Best Protocols; Protocol B: One step protocol for intracellular (nuclear) proteins).Excitation: 488 nm; Emission: 695 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|T-PET, T-bet, TBET, TBLYM, T-box expressed in T cells, T-box protein 21, T-box transcription factor TBX21, T-cell-specific T-box transcription factor T-bet, transcription factor TBLYM|
|eBio4B10 (4B10, 4-B10)|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
For Research Use Only.