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Thermo Scientific™ Tango™ HTR1D-bla U2OS Cells

Product Code. 10756189
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2 x 106 Cells
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10756189 2 x 106 Cells Each
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Product Code. 10756189 Brand Thermo Scientific™ Brand No. K1524

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Description

The Tango™ HTR1D-bla U2OS cells contain the human 5-Hydroxytryptamine (Serotonin) Receptor 1D (HTR1D) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line.

The Tango™ HTR1D-bla U2OS cells contain the human 5-Hydroxytryptamine (Serotonin) Receptor 1D (HTR1D) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin/TEV protease fusion protein and the beta-lactamase (bla) reporter gene under the control of a UAS response element.

The Tango™ HTR1D-bla U2OS cells cells have been functionally validated for Z' factor and EC50 concentrations of established ligands. In addition, Tango™ HTR1D-bla U2OS cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary for each product.

TRUSTED_SUSTAINABILITY

Specifications

Accession Number NM_000864
Assay Entry Cell-based beta-arrestin recruitment
Cell State Dividing Cells
Content And Storage LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol.

Includes:
• Tango™ HTR1D-bla U2OS cells
• Tango™ HTR1D-bla U2OS Assay Protocol
• Certificate of Analysis

The Tango™ HTR1D-bla U2OS cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
Cytoplasmic Tail Tail from AVPR2 GPCR
Description Tango™ HTR1D-bla U2OS Cells, 2 x 106 Cells, U2OS Cell Line, Fluorescent Detection Method, GPCRs Druggable Target, BLA (Beta-Lactamase) Reporter, Liquid Nitrogen Storage
Detection Method Fluorescence
For Use With (Application) Pharma and biopharma, drug discovery and development, target and lead identification and validation, G-protein coupled receptor biology, cell-based GPCR reporter assays
Gene Symbol HTR1D
Includes Tango™ HTR1D-bla U2OS cells (LiveBLAzer™-FRET B/G loading kit, solution D and other materials are required separately; please refer to the protocol). Includes: Tango™ HTR1D-bla U2OS cells, Tango™ HTR1D-bla U2OS assay protocol, certificate of analysis
Population Status Single Cell Clone Origin
Product Line Tango
Quantity 2 x 106 Cells
Reporter Gene BLA (Beta-Lactamase)
Sample Type U2OS
Shipping Condition Room Temperature
Target Entry HTR1D
Type U2OS Cell
Cell Line U2OS
System Type Tango™
Druggable Target GPCR
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For Research Use Only. Not for use in diagnostic procedures.

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