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Applied Biosystems™ TaqMan™ Advanced miRNA Human Endogenous Controls 96-well Plate, standard
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This product contains 1 plate than can be used to evaluate 30 potential endogenous control miRNAs for up to 3 samples. Two exogenous (non-human) controls are included for normalization of sample input.
Features of the TaqMan Advanced miRNA Human Endogenous Controls 96-Well Plate include:
• Reproducibility—pre-spotted assays are designed to reduce pipetting error and minimize experimental variability
• Sample savings—requires only 2 μL of purified plasma/serum or 1–10 ng of total RNA per sample
• Updated content—assay content updated for mirBase v.21
• Easy to use—just add a mix of diluted miR-Amp and master mix and run the qPCR; no additional pipetting required
• Gold-standard TaqMan qPCR chemistry—TaqMan Advanced miRNA assays draw on Thermo Fisher Scientific's industry-leading bioinformatics assay design pipeline and verification system to help ensure high specificity and minimal cross-reactivity, even for closely-related miRNA families
Product details:
Number of plates: 1
Number of unique miRNAs (not including exogenous controls): 30
Exogenous controls: cel-miR-39-3p, ath-miR159a
Compatible with the following Applied Biosystems qPCR instruments: Viia7, StepOnePlus, 7500/7500 Fast, QuantStudio 3, QuantStudio 5, QuantStudio 6, QuantStudio 7 Flex, and QuantStudio 12k Flex
Recommended master mix: TaqMan Fast Advanced Master Mix
Notes
• TaqMan Advanced miRNA plates require the use of the TaqMan Advanced cDNA Synthesis Kit, which uses a universal reverse transcription (RT) chemistry to prepare the cDNA template for use with TaqMan Advanced miRNA assays for detection and quantification of mature human miRNAs in biological samples.
• These plates are not compatible with the first-generation TaqMan MicroRNA cDNA Synthesis Kit, which uses an miRNA-specific reverse transcription chemistry.
Specifications
Specifications
| Content And Storage | Store at room temperature. |
| Detection Method | Primer-probe |
| Format | 96-Well Plate |
| GC-Rich PCR Performance | High |
| PCR Method | 2-step RT-qPCR |
| Reaction Speed | Standard |
| For Use With (Equipment) | 7500 System, 7900 HT System, QuantStudio™ 12 k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, ViiA™ 7 System, StepOnePlus™ System |
| Product Line | TaqMan |
| Product Type | Endogenous Control 96-Well Plate |
| Quantity | 1 plate |
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For Research Use Only. Not for use in diagnostic procedures.
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