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Thermo Scientific™ TMT10plex™ Isobaric Label Reagents and Kits

TMT10plex Isobaric Label Reagents and Kits contain ten isobaric compounds with the same mass and chemical structure (i.e., isotopomeric) composed of an amine-reactive NHS-ester group, a spacer arm, and a mass reporter.

Brand:  Thermo Scientific™ 90113

2738.62 EUR valid until 2024-03-29
Use promo code "21653" to get your promotional price.



Additional Details : Weight : 0.50000kg Transport : UN number : 3316 Chem class : 9 Pack group : III


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Product Code. 15295743

  • 3190.00€ / 30 reactions
Estimated Shipment: 15-04-2024
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Description

Description

TMT10plex Isobaric Label Reagents and Kits contain ten isobaric compounds with the same mass and chemical structure (i.e., isotopomeric) composed of an amine-reactive NHS-ester group, a spacer arm, and a mass reporter. Up to ten different peptide samples prepared from cells or tissues can be labeled in parallel and then combined for analysis. For each sample, a unique reporter mass (i.e., TMT10 126-131 Da) in the low-mass region of the high-resolution MS/MS spectrum is used to measure relative protein expression levels during peptide fragmentation and tandem mass spectrometry.

The Tandem Mass Tag (TMT) Isobaric Label reagents and kits are specifically designed to enable identification and quantitation of proteins in different samples using tandem mass spectrometry. The TMT10plex label reagents share an identical structure with TMTzero, TMTduplex, and TMTsixplex reagents, but contain different numbers and combinations of 13C and 15N isotopes in the mass reporter. The different isotopes result in a 10-plex set of tags that have mass differences in the reporter that can be detected using high resolution Orbitrap MS instruments.

Advantages of the TMT10plex label reagents include increased multiplex relative quantitation, increased sample throughput, and fewer missing quantitative values among samples. TMT10plex label reagents are ideal for analysis of multiple protein samples from inhibitor dose response experiments, time course experiments, or improving statistics on biological replicates.

Features of TMT10plex Isobaric Label reagent sets and mass tagging kits
Powerful—multiplexed MS analysis increases sample throughput and enables relative quantitation of up to ten different samples derived from cells, tissues, or biological fluids
Consistent—identical reagent structure and performance among TMTzero, TMTduplex, TMTsixplex, and TMT10plex reagents allows efficient transition from method development to multiplex quantitation, enabling biomarker discovery research across platforms and datasets
Robust—increased multiplex capability results in fewer missing quantitative values among samples and higher confidence among replicates
Efficient—amine-reactive, NHS-ester-activated reagents ensure efficient labeling of all peptides regardless of protein sequence or proteolytic enzyme specificity
Compatible—optimized for use with high resolution Orbitrap MS/MS platforms, with data analysis fully supported by Proteome Discoverer 2.3.

Applications:
• Protein identification and quantitation from multiple samples of cells, tissue, or biological fluids
• Protein expression profiling of normal vs. disease states or control vs. treated
• Quantitative analysis of proteins for which no antibodies are available
• Identification and quantitation of membrane and post-translationally modified proteins
• Identification and quantification of hundreds to thousands of proteins in a single experiment

TMT Reagents are provided as standalone sets or in optimized kit formats containing all necessary reagents and controls for maximum flexibility, convenience, and reliability. When combined with the industry-leading, high resolution Thermo Scientific Orbitrap instruments and software, TMT10plex reagents provide integrated total solutions for quantitative protein expression analysis.

Specifications

Specifications

TMT10plex™ Isobaric Mass Tagging Kit
Isobaric Mass Tag Labeling Kit
Sufficient for: Three 10-plex (3 x 10-way) experiments
• TMT10-126 Label Reagent, 3 x 0.8 mg
• TMT10-127N Label Reagent, 3 x 0.8 mg
• TMT10-127C Label Reagent, 3 x 0.8 mg
• TMT10-128N Label Reagent, 3 x 0.8 mg
• TMT10-128C Label Reagent, 3 x 0.8 mg
• TMT10-129N Label Reagent, 3 x 0.8 mg
• TMT10-129C Label Reagent, 3 x 0.8 mg
• TMT10-130N Label Reagent, 3 x 0.8 mg
• TMT10-130C Label Reagent, 3 x 0.8 mg
• TMT10-131 Label Reagent, 3 x 0.8 mg
• Dissolution Buffer (1M triethyl ammonium bicarbonate), 5 mL
• Denaturing Reagent (10% SDS), 1 mL
• Reducing Reagent (0.5M TCEP), 1 mL
• Iodoacetamide, 12 x 9 mg
• Quenching Reagent (50% hydroxylamine), 1 mL
• Pierce Trypsin Protease, MS Grade, 5 x 20 μg
• Trypsin Storage Solution, 250 μL
• Albumin, Bovine, 2.5 mg

Store at –20°C.

Cell Lysate
Mass Spectrometry
30 Reactions
Mass Spectrometer
TMT10plex™
Tandem Mass Tag™ (TMT) Labels
Protein
Protein Labeling
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Safety and Handling

Safety and Handling

Product Identifier
  • TMT10plex Isobaric Label Reagents and Kits

Signal Word
  • Danger

Hazard Category
  • Acute toxicity Category 3
  • Acute toxicity Category 4
  • ACUTE AQUATIC Acute 1
  • Carcinogenicity Category 1B
  • Carcinogenicity Category 2
  • Serious eye damage/eye irritation Category 1
  • Serious eye damage/eye irritation Category 2
  • Substances and mixtures corrosive to metals Category 1
  • Respiratory sensitiser Category 1
  • Skin corrosion/irritation Category 1 B
  • Skin corrosion/irritation Category 2
  • Skin sensitiser Category 1
  • Specific target organ toxicity after repeated exposure Category 2
  • Specific target organ toxicity Category 3

Hazard Statement
  • H290-May be corrosive to metals.
  • H301-Toxic if swallowed.
  • H302-Harmful if swallowed.
  • H312-Harmful in contact with skin.
  • H314-Causes severe skin burns and eye damage.
  • H315-Causes skin irritation.
  • H317-May cause an allergic skin reaction.
  • H318-Causes serious eye damage.
  • H319-Causes serious eye irritation.
  • H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
  • H335-May cause respiratory irritation.
  • H350-May cause cancer.
  • H351-Suspected of causing cancer.
  • H360-May damage fertility or the unborn child.
  • H373-May cause damage to organs.
  • H400-Very toxic to aquatic life.

Precautionary Statement
  • P201-Obtain special instructions before use.
  • P202-Do not handle until all safety precautions have been read and understood.
  • P234-Keep only in original container.
  • P260-Do not breathe dust/fume/gas/mist/vapours/spray.
  • P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
  • P264-Wash hands thoroughly after handling.
  • P270-Do not eat, drink or smoke when using this product.
  • P271-Use only outdoors or in a well-ventilated area.
  • P272-Contaminated work clothing should not be allowed out of the workplace.
  • P273-Avoid release to the environment.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P284-¢In case of inadequate ventilation! wear respiratory protection.
  • P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
  • P302+P352-IF ON SKIN: Wash with plenty of water/soap.
  • P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
  • P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
  • P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
  • P308+P313-IF exposed or concerned: Get medical advice/attention.
  • P310-Immediately call a POISON CENTER/doctor/.
  • P330-Rinse mouth.
  • P332+P313-If skin irritation occurs: Get medical advice/attention.
  • P333+P313-If skin irritation or rash occurs: Get medical advice/attention.
  • P337+P313-If eye irritation persists: Get medical advice/attention.
  • P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .
  • P362+P364-Take off contaminated clothing and wash it before reuse.
  • P363-Wash contaminated clothing before reuse.
  • P403+P233-Store in a well-ventilated place. Keep container tightly closed.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information
  • MIXTURE LIST-Contains : Trypsin, 2-Iodoacetamide, Hydroxylamine,Tris(2-carboxyethyl)phosphonium chloride, Sodium lauryl sul

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