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  6. Thermo Scientific™ Pierce™ Premium Grade BS3

Thermo Scientific™ Pierce™ Premium Grade BS3

Product Code. 15352627 Shop All Thermo Scientific Products
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100 mg
1 g
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100mg
1g
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Product Code. 15352627 Supplier Thermo Scientific™ Supplier No. PG82083

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Description

Crosslink proteins and other biomolecules in applications where product integrity and risk minimization are paramount with Thermo Scientific™ Pierce™ Premium Grade BS3, a high-quality formulation of this popular amine-reactive crosslinker.

Thermo Scientific Pierce Premium Grade BS3 is our highest quality formulation of this popular amine-reactive crosslinker, specially characterized for applications where product integrity and risk minimization are paramount. BS3 is a noncleavable, membrane-impermeable, water-soluble crosslinking agent for amines.

  • High quality - identity and purity confirmed by multiple tests, including quantitative NMR
  • Product integrity - enhanced level of testing and characterization compared to standard grade
  • Lot retention - ample supply of past lots retained to help ensure future process testing
  • Change management - Change Control Notification (CCN) service
  • Consistent manufacture - batch-specific manufacturing documentation review
Compared to the standard grade product, Premium Grade BS3 provides more clearly defined quality and product support by including increased analytical testing and product characterization, greater batch-specific information and quality assurance review, extensive lot sample retention, and change control notification.
BS3 is bis(sulfosuccinimidyl)suberate, an amine-to-amine crosslinker that is homobifunctional, water-soluble, non-cleavable, and membrane impermeable. BS3 contains an amine-reactive N-hydroxysulfosuccinimide (NHS) ester at each end of an 8-carbon spacer arm. NHS esters react with primary amines at pH 7-9 to form stable amide bonds, along with release of the N-hydroxysulfosuccinimide leaving group. Proteins, including antibodies, generally have several primary amines in the side chain of lysine (K) residues and the N-terminus of each polypeptide that are available as targets for NHS-ester crosslinking reagents.
Applications:
  • Crosslink cell-surface proteins prior to cell lysis and immunoprecipitation
  • Identify receptor-ligand interactions by chemical crosslinking
  • “Fix” protein interactions to allow identification of weak or transient protein interactions
  • Identify near-neighbor protein interactions
  • Crosslink proteins to create bioconjugates via single-step reactions
  • Immobilize proteins onto amine-coated surfaces

TRUSTED_SUSTAINABILITY

Specifications

For Use With (Application) Protein labeling and crosslinking, crosslink cell-surface proteins prior to cell lysis and immunoprecipitation, identify receptor-ligand interactions by chemical crosslinking, fix protein interactions to allow identification of weak or transient protein
Formulation Bis(sulfosuccinimidyl) suberate; powder solid
Kit Contents Premium Grade bis(sulfosuccinimidyl) suberate powder
Molecular Weight (g/mol) 572.43
Sufficient For 175mL of typical 1X (1mM) reaction solution, or approx. 50 uses at 2mg reagent per use
Spacer Arm Length Mid-length
Molecular Formula C16H18N2Na2O14S2
Content And Storage Store with desiccant at 4°C
Product Line Pierce
Form Powder
Quantity 100 mg
Solubility Water
Product Type BS3
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