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Thermo Scientific™ Pierce™ Premium Grade BS3
Crosslink proteins and other biomolecules in applications where product integrity and risk minimization are paramount with Thermo Scientific™ Pierce™ Premium Grade BS3, a high-quality formulation of this popular amine-reactive crosslinker.
Brand: Thermo Scientific™ PG82083
Additional Details : Weight : 0.01000kg
Description
Thermo Scientific Pierce Premium Grade BS3 is our highest quality formulation of this popular amine-reactive crosslinker, specially characterized for applications where product integrity and risk minimization are paramount. BS3 is a noncleavable, membrane-impermeable, water-soluble crosslinking agent for amines.
- High quality - identity and purity confirmed by multiple tests, including quantitative NMR
- Product integrity - enhanced level of testing and characterization compared to standard grade
- Lot retention - ample supply of past lots retained to help ensure future process testing
- Change management - Change Control Notification (CCN) service
- Consistent manufacture - batch-specific manufacturing documentation review
BS3 is bis(sulfosuccinimidyl)suberate, an amine-to-amine crosslinker that is homobifunctional, water-soluble, non-cleavable, and membrane impermeable. BS3 contains an amine-reactive N-hydroxysulfosuccinimide (NHS) ester at each end of an 8-carbon spacer arm. NHS esters react with primary amines at pH 7-9 to form stable amide bonds, along with release of the N-hydroxysulfosuccinimide leaving group. Proteins, including antibodies, generally have several primary amines in the side chain of lysine (K) residues and the N-terminus of each polypeptide that are available as targets for NHS-ester crosslinking reagents.
Applications:
- Crosslink cell-surface proteins prior to cell lysis and immunoprecipitation
- Identify receptor-ligand interactions by chemical crosslinking
- “Fix” protein interactions to allow identification of weak or transient protein interactions
- Identify near-neighbor protein interactions
- Crosslink proteins to create bioconjugates via single-step reactions
- Immobilize proteins onto amine-coated surfaces
Specifications
Pierce™ Premium Grade BS3, 100mg, Powder, Mid-Length Spacer, >300°C Melting Point, Chemical Labeling Method, 4°C Storage, For Protein Labeling, Crosslinking and Identify Near-Neighbor Protein Interactions | |
Bis(sulfosuccinimidyl) suberate; powder solid | |
572.43 | |
Mid-length | |
Store with desiccant at 4°C | |
Water |
Protein labeling and crosslinking, crosslink cell-surface proteins prior to cell lysis and immunoprecipitation, identify receptor-ligand interactions by chemical crosslinking, fix protein interactions to allow identification of weak or transient protein | |
Premium Grade bis(sulfosuccinimidyl) suberate powder | |
175mL of typical 1X (1mM) reaction solution, or approx. 50 uses at 2mg reagent per use | |
C16H18N2Na2O14S2 | |
100 mg | |
BS3 (bis(sulfosuccinimidyl)suberate) |