Learn More
Thermo Scientific™ Pierce™ Premium Grade BS3
Crosslink proteins and other biomolecules in applications where product integrity and risk minimization are paramount with Thermo Scientific™ Pierce™ Premium Grade BS3, a high-quality formulation of this popular amine-reactive crosslinker.
Brand: Thermo Scientific™ PG82084
Additional Details : Weight : 0.01000kg
Description
Thermo Scientific Pierce Premium Grade BS3 is our highest quality formulation of this popular amine-reactive crosslinker, specially characterized for applications where product integrity and risk minimization are paramount. BS3 is a noncleavable, membrane-impermeable, water-soluble crosslinking agent for amines.
- High quality - identity and purity confirmed by multiple tests, including quantitative NMR
- Product integrity - enhanced level of testing and characterization compared to standard grade
- Lot retention - ample supply of past lots retained to help ensure future process testing
- Change management - Change Control Notification (CCN) service
- Consistent manufacture - batch-specific manufacturing documentation review
BS3 is bis(sulfosuccinimidyl)suberate, an amine-to-amine crosslinker that is homobifunctional, water-soluble, non-cleavable, and membrane impermeable. BS3 contains an amine-reactive N-hydroxysulfosuccinimide (NHS) ester at each end of an 8-carbon spacer arm. NHS esters react with primary amines at pH 7-9 to form stable amide bonds, along with release of the N-hydroxysulfosuccinimide leaving group. Proteins, including antibodies, generally have several primary amines in the side chain of lysine (K) residues and the N-terminus of each polypeptide that are available as targets for NHS-ester crosslinking reagents.
Applications:
- Crosslink cell-surface proteins prior to cell lysis and immunoprecipitation
- Identify receptor-ligand interactions by chemical crosslinking
- “Fix” protein interactions to allow identification of weak or transient protein interactions
- Identify near-neighbor protein interactions
- Crosslink proteins to create bioconjugates via single-step reactions
- Immobilize proteins onto amine-coated surfaces
Specifications
Amine-Amine | |
BS3 | |
No | |
Upon receipt store with desiccant at 4°C. Sufficient for 1.74 L of typical 1X (1 mM) reaction solution or for ∼500 uses at 2 mg reagent per use. |
|
Ambient | |
Chemical Labeling | |
Sulfo-NHS Ester | |
Powder | |
Water | |
Yes |
No | |
572.43 | |
11.4 Å | |
No | |
Pierce™ | |
Homobifunctional | |
Medium (10 to 30 Å) | |
1 g | |
Standard, Single-use, Premium-grade | |
Crosslinker |