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Novus Biologicals™ TNF-alpha Antibody Pair [HRP]

Artikelnummer. p-7183036 Shop alle Bio Techne produkter
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Quantity:
15 Plates
5 Plates
Pakningsstørrelse:
Pack of 15
Pack of 5
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Artikelnummer. 18207446

Brand: Novus Biologicals™ NBP27939015PLATES

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Monoclonal Antibody Pair

This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Ferret TNF-alpha.
TRUSTED_SUSTAINABILITY

Tekniske data

Assay Range 156.25 pg/mL
Assay Sensitivity 156.25-5000 pg/mL
Assay Sandwich ELISA
Conjugate HRP
Product Type Antibody Pairs
Specificity This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Ferret TNF-alpha.
Target Species Ferret
For Use With (Application) Sandwich ELISA
Gene Alias APC1 protein, Cachectin, DIF, TNF, monocyte-derived, TNF-a, TNF-alphacachectin, TNFATNF, macrophage-derived, TNFSF2TNF superfamily, member 2, tumor necrosis factor, tumor necrosis factor (TNF superfamily, member 2), tumor necrosis factor alpha, Tumor necrosis factor ligand superfamily member 2, tumor necrosis factor-alpha
Gene ID (Entrez) 7124
Gene Symbol TNF
Host Species Mouse/Rabbit
Kit Contents
  • Standard: Each vial contains 70 ng of recombinant TNF-alpha. Reconstitute with 1 mL detection antibody dilution buffer.
  • Capture Antibody: 0.5 mg/mL of rabbit anti-TNF-alpha monoclonal antibody (in PBS, pH 7.4).
  • Detection Antibody: 0.2 mg/mL mouse anti-TNF-alpha monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4C).
  • Quantity 15 Plates
    Regulatory Status RUO
    Research Discipline Adaptive Immunity, Asthma, Autophagy, Biologically Active Proteins, Cancer, Cytokine Research, Diabetes Research, Immune System Diseases, Immunology, Innate Immunity
    Storage Requirements Storage is content dependent.
    Target TNF-alpha
    Technique 1. Capture Antibody: Dilute to a working concentration of 2 μg/mL in PBS before coating. 2. Detection Antibody: Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use. 3. Standard: After reconstitution, store at -20 to -80°C in a manual defrost freezer. A six-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 5 ng/mL is recommended.
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