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TCR V alpha 2 Monoclonal Antibody (B20.1), eFluor™ 450, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 48-5812-80

Additional Details : Weight : 0.25000kg

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Product Code. 15361790

This item has been discontinued and is no longer available. View the product for possible alternatives or contact our Technical Support team on 056 260 260 for assistance.
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Description

Description

Description: The B20.1 monoclonal antibody reacts with mouse T-cell receptor (TCR) V alpha 2 chains. This antibody recognizes the majority of the TCR V alpha 2 subfamily in mice carrying the a, b and c haplotypes. The antibody also reacts with the products of T cell receptor, V delta 8 due to the high degree of homology. Applications Reported: This B20.1 antibody has been reported for use in flow cytometric analysis. Applications Tested: This B20.1 antibody has been tested. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome. Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser. Filtration: 0.2 μm post-manufacturing filtered.

The ability of T cell receptors (TCR) to discriminate foreign from self-peptides presented by major histocompatibility complex (MHC) class II molecules is essential for an effective adaptive immune response. TCR recognition of self-peptides has been linked to autoimmune disease. Mutant self-peptides have been associated with tumors. Engagement of TCRs by a family of bacterial toxins know as superantigens has been responsible for toxic shock syndrome. Autoantibodies to V beta segments of T cell receptors have been isolated from patients with rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE). The autoantibodies block TH1-mediated inflammatory autodestructive reactions and are believed to be a method by which the immune system compensates for disease. Most human T cells express the TCR alpha-beta and either CD4 or CD8 molecule (single positive, SP). A small number of T cells lack both CD4 and CD8 (double negative, DN). Increased percentages of alpha-beta DN T cells have been identified in some autoimmune and immunodeficiency disorders. Gamma-delta T cells are primarily found within the epithelium. They show less TCR diversity and recognize antigens differently than alpha-beta T cells. Subsets of gamma-delta T cells have shown antitumor and immunoregulatory activity.
Specifications

Specifications

TCR V alpha 2
Monoclonal
0.2 mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
Rat
25 μg
Primary
4° C, store in dark, DO NOT FREEZE!
Liquid
Flow Cytometry
B20.1
eFluor 450
TCRVA; TCRVA2; V alpha 2; V alpha-2 TCR; Valpha 2; Valpha2; Va 2; Va2
Affinity chromatography
RUO
Mouse
Antibody
IgG2a λ
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For Research Use Only.