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Invitrogen™ Human Titin (aa 1022-1155) Control Fragment Recombinant Protein

Product Code. 30204783
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Quantity:
100 μL
Unit Size:
100µL
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Product Code. 30204783

Brand: Invitrogen™ RP89761

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This item is not returnable. View return policy

Description

Recombinant Protein

Highest antigen sequence indentity to the following orthologs: Mouse (93%), Rat (93%). This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-52379 (PA5-52379. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.

Monoclonal Anti-Titin can be used for study of the elastic filaments within sarcomeric structures. It is also useful as a differentiation marker in the separation of rhabdomyosarcomas from other muscle tumors. By indirect immunofluorescence the antibody displays a typical striated staining pattern on frozen sections of chicken skeletal and cardiac muscle tissues. Stains the region of the A-I junction by indirect immunofluorescence. It shows a decoration line 0.05 mm from the end of the A band in electron micro-graphs. In immunoblotting using total extracts of chicken breast muscle the antibody reacts specifically with both bands of the titin doublet, and showed no reaction with nebulin.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number Q8WZ42
Concentration ≥5.0 mg/mL
For Use With (Application) Blocking Assay, Control
Formulation 1 M urea, PBS with no preservative; pH 7.4
Gene ID (Entrez) 7273
Name Human Titin (aa 1022-1155) Control Fragment
Quantity 100 μL
Regulatory Status RUO
Gene Alias 1100001C23Rik; 2.7.11.1; 2310036G12Rik; 2310057K23Rik; 2310074I15Rik; AF006999; AV006427; CMD1G; CMH9; CMPD4; Connectin; connectin/titin; D330041I19Rik; D830007G01Rik; DKFZp451N061; EOMFC; FLJ26020; FLJ26409; FLJ32040; FLJ34413; FLJ39564; FLJ43066; HMERF; L56; LGMD2J; mdm; MYLK5; rhabdomyosarcoma antigen MU-RMS-40.14; shru; structural muscle protein titin; titin; titin protein homolog; titin sequence3; TMD; TTN
Common Name Titin
Gene Symbol TTN
Conjugate Unconjugated
Species Human
Recombinant Recombinant
Protein Tag His-ABP-tag
Sequence GTVSTSCYLAVQVSEEFEKETTAVTEKFTTEEKRFVESRDVVMTDTSLTEEQAGPGEPAAPYFITKPVVQKLVEGGSVVFGCQVGGNPKPHVYWKKSGVPLTTGYRYKVSYNKQTGECKLVISMTFADDAGEYT
Content And Storage -20°C, Avoid Freeze/Thaw Cycles
Expression System E. coli
Form Liquid
Purity or Quality Grade >80% by SDS-PAGE and Coomassie blue staining
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