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Vimentin, eFluor 570, clone: V9, eBioscience™

Mouse Monoclonal Antibody

Marque:  Affymetrix eBioscience 41-9897-80

Informations supplémentaires : Poids : 0.25000kg

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Code produit. 15361080

  • 98.25€ / 25µg


Date dexpédition estimée
du fournisseur 24-07-2018
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Description

Description

Description: The V9 monoclonal antibody recognizes human Vimentin, a 57kDa protein that functions as a structural component of intermediate filaments. Vimentin is expressed in cells derived from the mesenchyme but also in specific populations such as radial glia and immature glial cells, pancreatic precursor cells. It is proposed to be a marker of cardiac differentiation. In neural cells, vimentin expression is gradually replaced by neurofilaments. Reports have also shown surface expression of vimentin on activated macrophages, platelets, as well as apoptotic T cells and neutrophils.This antibody also recognizes canine (dog), rat and chicken vimentin but does not recognize mouse vimentin.Applications Reported: This V9 antibody has been reported for use in immunocytochemistry and immunohistochemical staining of frozen (IHC-F) and formalin-fixed paraffin embedded tissue sections (IHC-P).Applications Tested: This V9 antibody has been tested immunocytochemistry on fixed and permeabilized C6 cells at less than or equal to 5 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Filter Recommendation: When using this eFluor® 570 antibody conjugate, we recommend a filter that will capture the 570 emission wavelength (for example, Excitation 545/25, 565LP, Emission 605/70). A standard Alexa Fluor® 555 or TRITC filter is acceptable.Excitation: 555 nm; Emission: 570 nm
Spécification

Spécification

Vimentin
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P31000, P09654, P08670
VIM, FLJ36605
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
CanineþChickenþHumanþRat
ImmunocytochemistryþImmunohistochemistryþImmunohistochemistry (Frozen)
V9
eFluor 570
VIM
Conjugated
VIM
IgG1, kappa
25μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
7431
Vidéos

For Research Use Only.