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Thermo Scientific™ Vivid™ Cyan Fluorescent Standard

Product Code. 10032952
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Product Code. 10032952 Supplier Thermo Scientific™ Supplier No. P2877

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Description

Vivid™ Fluorescent Standards represent the products formed during oxidation of Vivid™ Substrates by cytochrome P450 enzymes. These standards enable quantification of enzyme activity when used as part of the Vivid™ CYP450 Screening Kits.

Vivid™ Fluorescent Standards represent the products formed during oxidation of Vivid™ Substrates by cytochrome P450 enzymes. These standards enable quantification of enzyme activity when used as part of the Vivid™ CYP450 Screening Kits. Vivid™ Fluorescent Standards are included in the Vivid™ Screening Kits, but may also be purchased separately. They are supplied as a dried film.

More About Vivid™ CYP450 Screening Kits

Vivid™ CYP450 Screening Kits are high-throughput fluorescence-based assays for detection of enzyme-drug interactions and CYP450 inhibition. Vivid™ CYP450 Screening Kits include Vivid™ Substrate, Vivid™ Fluorescent Standard, reaction buffer, Vivid™ Regeneration System, NADP+, and CYP450 BACULOSOMES™ Plus Reagent. Vivid™ Fluorogenic Substrates are blocked dyes that yield minimal fluorescence signal until cleaved or hydroxylated. Oxidation at either of two potential sites releases the highly fluorescent product. CYP450 BACULOSOMES™ Plus Reagents are microsomes prepared from insect cells expressing a human CYP450 isozyme and human cytochrome P450 reductase. CYP450 BACULOSOMES™ Plus Reagents offer a distinct advantage over human liver microsomes in that only one CYP450 isozyme is expressed, thereby preventing metabolism by other CYP450s.

Applications

  • High-throughput screening of enzyme-drug interactions
  • Compound profiling for drug inhibition of cytochrome P450 isozymes
  • Generation of predictive SAR models to guide compound acquisition
TRUSTED_SUSTAINABILITY

Specifications

Description Vivid™ Cyan Fluorescent St and ard, 1 Each, Powder, 0.1μM Concentration, 584.52 Molecular Weight, –20°C Storage Requirements, Used for Cell Viability, Proliferation and Function and Pharma and Biopharma and Protein Assays and Analysis a
Formulation Powder
Concentration 0.1 μm
Format Powder
For Use With (Application) Cell viability, proliferation and function and pharma and biopharma and protein assays and analysis and target-based adme/tox assays, protein biology, cell analysis, proliferation and function, cell metabolism, drug discovery and development, target-based
Quantity Each
Product Type Fluorescent Standard
Kit Contents Vivid™ CYP450 Fluorogenic substrates, BACULOSOMES™ CYP450 reagents, fluorescent standards and an NADPH-regeneration system. Vivid™ CYP450 Fluorogenic substrates are also available separately. Store reaction buffers at 4°C or room tem
Content And Storage • Vivid™ CYP450 Fluorogenic substrates
• BACULOSOMES™ CYP450 reagents
• Fluorescent standards
• NADPH-regeneration system.

Vivid™ CYP450 Fluorogenic substrates are also available separately.

Store reaction buffers at +4°C or room temperature. Store BACULOSOMES™, the NADPH-regeneration system, and NADP+ at -80°C. Store the Vivid™ substrates and standards at +4°C, protected from light. (For long-term storage, resuspend in organic solvent and keep at -20°C.)
Shipping Condition Wet Ice
Label or Dye Other Label(s) or Dye(s)
Product Line VIVID
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For Research Use Only. Not for use in diagnostic procedures.

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