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Zap-70 Mouse anti-Human, Mouse, PE, Clone: 1E7.2, eBioscience™

Mouse Monoclonal Antibody

Marque:  Affymetrix eBioscience 12-6695-82

Informations supplémentaires : Poids : 0.25000kg

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Code produit. 15279259

  • 181.00€ / 100µg


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Description

Description

Applications Tested: This 1E7.2 antibody has been tested by intracellular flow cytometric analysis of mouse thymocytes and human Jurkat cells using the Foxp3/Transcription Factor Buffer Set (cat. 00-5523) and protocol. Please refer to Best Protocols: Protocol B: One step protocol for (nuclear) intracellular proteins. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 μm post-manufacturing filtered. ZAP70, a 70 kDa member of the Syk tyrosine kinase family, plays a central role in lymphocyte activation and development, and is implicated in several immune disorders. Upon T cell antigen receptor (TCR) engagement, ZAP70 is phosphorylated on tyrosines 292, 315 and 319 in the interdomain B, located between the SH2 and kinase domains. Phosphorylation of both tyrosines 315 (a Vav binding site) and 319 (a Lck binding site) enhances ZAP70 function in mediating lymphocyte signaling, while tyrosine 292 terminates the transient activation of ZAP70 and attentuates lymphocyte signaling.
Spécification

Spécification

ZAP-70
Monoclonal
0.2 mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P43403, P43404
zeta-chain (TCR) associated protein kinase 70kDa
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
Human, Mouse
Flow Cytometry
1E7.2
PE
ZAP70
Liquid
ZAP70
IgG1, kappa
100 μg
4° C, store in dark, DO NOT FREEZE!
Primary
22637, 7535
Vidéos
Documents

For Research Use Only.