PCR Equipment and Supplies
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Invitrogen™ Platinum™ SuperFi II DNA Polymerase
Invitrogen Platinum SuperFi II DNA Polymerase is a proofreading DNA polymerase that combines superior fidelity with an innovative buffer, enabling universal primer annealing for the highest success in PCR.
| Shipping Condition | Dry Ice |
|---|---|
| Polymerase | Platinum SuperFi II DNA Polymerase |
| GC-Rich PCR Performance | High |
| Fidelity (vs. Taq) | >300X |
| Size (Final Product) | 20 kb or less |
| Reaction Format | Standalone |
| Reaction Speed | Fast |
| Hot Start | Built-In Hot Start |
| Overhang | Blunt |
| For Use With (Application) | Hot-start PCR,High-fidelity PCR |
Invitrogen™ SuperScript™ IV First-Strand Synthesis System
The SuperScript IV First-Strand Synthesis System for RT-PCR, available with or without ezDNase, is optimized for synthesis of first-strand cDNA from purified poly(A)+ or total RNA.
| GC-Rich PCR Performance | High |
|---|---|
| Format | Kit |
| Reverse Transcriptase | SuperScript IV |
| Size (Final Product) | Up to 12.3 kb |
| Reaction Format | Separate components |
| Final Product Type | First-Strand cDNA |
| Reaction Speed | 10 min. |
| Reagent Type | Reverse Transcription |
| Quantity | Each |
| Optimal Reaction Temperature | 50°C |
| Technique | Reverse Transcription |
| Starting Material | RNA |
Invitrogen™ dNTP Set (100 mM)
Invitrogen 100 mM dNTP Mix is a mixture of four nucleotides (dATP, dCTP, dGTP, dTTP) in purified water. Each nucleotide is at a concentration of 100 mM.
| Shipping Condition | Approved for shipment on Wet or Dry Ice |
|---|---|
| Form | Liquid |
| Label or Dye | Unlabeled |
| Concentration | 100 mM |
| For Use With (Application) | Standard PCR,RT-PCR,Real Time PCR (qPCR) |
Applied Biosystems™ TaqMan™ Gene Expression Master Mix
Precise and reliable real-time qPCR quantification over 9 logs of linear dynamic range with Applied Biosystems™ TaqMan™ Gene Expression Master Mix.
| Polymerase | AmpliTaq Gold DNA Polymerase |
|---|---|
| Purity or Quality Grade | UP (Ultra Pure) |
| Passive Reference Dye | ROX (Pre-mixed) |
| For Use With (Equipment) | 7000 System, 7300 System, 7500 System, 7900HT System, Applied Biosystems StepOnePlus™ Fast Real-Time PCR SystemStepOne™, Standard Mode, StepOnePlus™, Standard Mode |
| Sample Type | DNA (Genomic), cDNA |
| Reaction Speed | Standard |
| For Use With (Application) | Gene Expression |
| PCR Method | qPCR |
| Product Line | TaqMan |
| Detection Method | Primer-probe |
Applied Biosystems™ PowerUp™ SYBR™ Green Master Mix for qPCR
Pre-formulated, optimized, universal 2X master mix for real-time PCR workflows.
| Shipping Condition | Wet Ice |
|---|---|
| Concentration | 2X |
| For Use With (Application) | Gene Expression |
| PCR Method | qPCR |
| Multiplex Capability | Single-plex |
| Detection Method | SYBR |
| Includes | dUTP, UNG/UDG |
| Thermostability | 72 hr., Assembled Reaction |
| Polymerase | Dual-Lock Taq DNA Polymerase |
| Format | Tube |
| Product Type | Real Time PCR SYBR Master Mix |
| Passive Reference Dye | ROX (Pre-mixed) |
| For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT System, QuantStudio 12k Flex, QuantStudio 3, QuantStudio 5, QuantStudio 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio 7 Flex, StepOne, StepOnePlus, ViiA 7 System, Roche LightCycler PRO System, Bio-Rad CFX Opus 96 Real-Time PCR System |
| Sample Type | DNA |
| Reaction Speed | Fast or Standard |
| Inhibitor Tolerance (Simple) | Medium |
| Target Specificity | cDNA or gDNA only |
| Product Line | PowerUP, SYBR |
Thermo Scientific™ RevertAid H Minus Reverse Transcriptase (200 U/μL)
Thermo Scientific RevertAid H Minus Reverse Transcriptase is a recombinant M-MuLV RT. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity, but lacks RNase H activity due to a point mutation in the RNase H domain.
| Shipping Condition | Dry Ice |
|---|---|
| Content And Storage | • RevertAid H Minus Reverse Transcriptase (200 U/μL) |
| Reverse Transcriptase | RevertAid H Minus |
| Final Product Type | First-Strand cDNA |
| Concentration | 200 U/μL |
| Format | Stand-alone Enzyme |
| Size (Final Product) | Up to 13 kb |
| Reaction Format | Separate components |
| Reaction Speed | 60 min. |
| Reagent Type | Reverse Transcription |
| Ribonuclease H Activity | Reduced |
| Optimal Reaction Temperature | 42°C to 45°C |
| Technique | Reverse Transcription |
| Starting Material | RNA |
Thermo Scientific™ Phusion™ HF and GC Buffer Packs
5X Phusion Green buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel.
| Quantity | 4 x 1.5 mL |
|---|---|
| For Use With (Application) | Standard PCR |
Invitrogen™ Taq DNA Polymerase, recombinant
Taq DNA Polymerase is a thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer.
| Shipping Condition | Dry Ice |
|---|---|
| GC-Rich PCR Performance | Low |
| Fidelity (vs. Taq) | 1X |
| Overhang | 3'-A |
| Concentration | 5 U/μL |
| For Use With (Application) | Standard PCR |
| PCR Method | qPCR,Standard PCR |
| Exonuclease Activity | 5' - 3' |
| Polymerase | Taq DNA Polymerase |
| Format | Stand-alone enzyme |
| Size (Final Product) | 5 kb or less |
| Reaction Format | Separate Components |
| Product Type | DNA Polymerase |
| Reaction Speed | Standard |
| Hot Start | No |
| Starting Material | DNA |
Thermo Scientific™ RevertAid Reverse Transcriptase (200 U/μL)
Thermo Scientific RevertAid Reverse Transcriptase (RT) is a recombinant M-MuLV RT. It differs from the M-MuLV RT by its structure and catalytic properties.
| Shipping Condition | Dry Ice |
|---|---|
| Content And Storage | • RevertAid Reverse Transcriptase (200 U/μL) |
| Reverse Transcriptase | RevertAid |
| Final Product Type | First-Strand cDNA |
| Concentration | 200 U/μL |
| Format | Stand-alone Enzyme |
| Size (Final Product) | Up to 13 kb |
| Reaction Format | Separate components |
| Reaction Speed | 60 min. |
| Reagent Type | Reverse Transcription |
| Ribonuclease H Activity | Yes |
| Optimal Reaction Temperature | 42°C |
| Technique | Reverse Transcription |
| Starting Material | RNA |
Thermo Scientific™ dATP Solution (100 mM)
Thermo Scientific dATP (2'-deoxyadenosine 5'-triphosphate) is highly stable nucleotide, supplied as 100 mM aqueous solution titrated to pH 7.3-7.5 with NaOH.
Thermo Scientific™ DreamTaq DNA Polymerase (5 U/μL)
Thermo Scientific DreamTaq DNA Polymerase is an enhanced Taq DNA polymerase optimized for all standard PCR applications. It ensures higher sensitivity, longer PCR products, and higher yields compared to conventional Taq DNA polymerase.
| Polymerase | DreamTaq DNA Polymerase |
|---|---|
| GC-Rich PCR Performance | Low |
| Fidelity (vs. Taq) | 1X |
| Reaction Speed | Standard |
| Hot Start | No |
| Overhang | 3'-A |
| Concentration | 5 U/μL |
Invitrogen™ dNTP Mix (10 mM ea)
Invitrogen 10 mM dNTP Mix includes four nucleotides (dATP, dCTP, dGTP, dTTP) in 0.6 mM Tris-HCl. Each nucleotide is at a concentration of 10 mM and this mix is suitable for use in a wide variety of molecular processes.
| Shipping Condition | Approved for shipment on Wet or Dry Ice |
|---|---|
| Form | Liquid |
| Label or Dye | Unlabeled |
| Concentration | 10 mM |
| For Use With (Application) | Standard PCR,RT-PCR,Real Time PCR (qPCR) |
Invitrogen™ Platinum™ SuperFi II PCR Master Mixes
Invitrogen Platinum SuperFi II PCR Master Mix (2X) is a ready-to-use mixture of Platinum SuperFi II DNA Polymerase, Platinum SuperFi II Buffer, and dNTPs for convenient PCR setup and efficient amplification.
| Shipping Condition | Dry Ice |
|---|---|
| Polymerase | Platinum SuperFi II DNA Polymerase |
| GC-Rich PCR Performance | High |
| Fidelity (vs. Taq) | >300X |
| Size (Final Product) | 20 kb or less |
| Reaction Format | SuperMix or Master Mix |
| Product Type | PCR Master Mix |
| Reaction Speed | Fast |
| Hot Start | Built-In Hot Start |
| Overhang | Blunt |
| Concentration | 2X |
| For Use With (Application) | Hot-start PCR,High-fidelity PCR |
Thermo Scientific™ RevertAid H Minus First Strand cDNA Synthesis Kit
Thermo Scientific RevertAid H Minus First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates.
| Shipping Condition | Dry Ice |
|---|---|
| Format | Kit |
| Reverse Transcriptase | RevertAid H Minus |
| Size (Final Product) | Up to 13 kb |
| Reaction Format | Separate components |
| Final Product Type | First-Strand cDNA |
| Reaction Speed | 60 min. |
| Reagent Type | Reverse Transcription |
| Ribonuclease H Activity | Reduced |
| Optimal Reaction Temperature | 42°C to 45°C |
| Technique | Reverse Transcription |
| Starting Material | RNA |
| For Use With (Application) | Real Time PCR (qPCR),RT-PCR |
Applied Biosystems™ resDNASEQ™ Quantitative E. coli DNA Kits
This quantitative PCR (qPCR)-based kit, part of the resDNASEQ™ E. coli Residual DNA Quantitation System, enables highly sensitive quantitation of host cell DNA from E. coli expression systems, typically in less than 4 hours.
| No. of Reactions | 100 Reactions |
|---|---|
| Format | Kit |
| Label or Dye | FAM |
| Quantity | 100 reactions |
| Host Cell | E.coli |
| PCR Method | qPCR |
| Product Line | resDNASEQ |
| Detection Method | Primer-probe |