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Applied Biosystems™ RNA UltraSense™ One-Step Quantitative RT-PCR System

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Quantity:
100 rxns
Unit Size:
100 reactions
Product Code. Quantity unitSize
10616743 100 rxns 100 reactions
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Product Code. 10616743

Brand: Applied Biosystems™ 11732927

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Description

RNA UltraSense™ One-Step Quantitative RT-PCR System

The RNA UltraSense™ One-Step Quantitative RT-PCR System is specially designed for amplification and real-time quantification of ultra-low abundance transcripts and RNA viruses. The optimized, ultra-concentrated system combines SuperScript™ III RT and Platinum™ Taq DNA Polymerase to provide greater priming specificity, higher product yields, and detection over a broad dynamic range. The high concentration formulation provides great flexibility with low concentration / high volume RNA samples, and improves performance with targets rich in secondary structure. As little as 25 pg/μl total RNA can be detected with a PCR efficiency of 97% (Figure 1). The RNA UltraSense™ System features:

  • SuperScript™ III RT for higher temperature cDNA synthesis (up to 60°C) for greater success with RNA secondary structure
  • Platinum™ Taq DNA Polymerase with hot-start technology for improved specificity
  • Optimized performance with either LUX™ Fluorogenic Primers or dual-labeled fluorogenic probes
  • Proprietary, ultra-concentrated buffer for addition of large sample volumes (up to 70%) and success with RNA secondary structure
  • Validated performance on multiple real-time instrument platforms
  • One-step format for speed, convenience, and less reaction-to-reaction variability

    Applications:
    Real-time quantitative amplification of RNA, including:
  • RNA viruses
  • Low-abundance RNA transcripts
  • Difficult-to-amplify RNA targets
  • High-value RNA detection and research
    • TRUSTED_SUSTAINABILITY

    Specifications

    Content And Storage The RNA UltraSense™ One-Step Quantitative RT-PCR System is supplied with the following components: 250 μl RNA UltraSense™ Enzyme Mix (includes SuperScript™ III RT, Platinum™ Taq DNA Polymerase, and RNaseOUT™ Ribonuclease Inhibitor); 2 x 1 ml RNA UltraSense™ 5X Reaction Mix; 300 μl 20X Bovine Serum Albumin (BSA), Ultrapure Molecular Biology Grade (5 mg/ml); 1 ml 50-mM magnesium sulfate (MgSO4 ); 100 μl ROX Reference Dye. Sufficient reagents are provided for 100 reactions. Store components at -20°C. Stability can be extended by storing at -80°C. ROX Reference Dye must be stored in the dark.
    Detection Method Primer-probe
    GC-Rich PCR Performance High
    PCR Method 1-step RT-qPCR
    Polymerase DNA Polymerase
    Reaction Speed Standard
    Product Line Platinum, SuperScript, UltraSense
    Product Type One-Step Quantitative RT-PCR System
    Quantity 100 rxns
    Shipping Condition Dry Ice
    Sufficient For 100 Reactions
    No. of Reactions 100 Reactions
    Sample Type RNA
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    For Research Use Only. Not for use in diagnostic procedures.

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