Real Time PCR Master Mixes

Real-time PCR master mixes are batch mixtures of PCR reagents at optimal concentrations that can be prepared and divided among multiple PCR tubes/plates. They reduce pipetting and risk of contamination, save time, and limit possible mixing errors.

1 – 15 of 94 results

Applied Biosystems™ PowerTrack™ SYBR Green Master Mix for qPCR

PowerTrack SYBR Green Master Mix is a pre-formulated, optimized, universal 2X master mix for real-time PCR workflows with built-in visual dye–based indicators for accurate reaction set up.

Pricing and Availability
Specifications

Shipping Condition	Dry Ice
Content And Storage	• PowerTrack SYBR Green Master Mix • 40X Yellow Sample Buffer Store at -20±5°C in the dark.
InhibitorToleranceSimple	High
Concentration	2X
For Use With (Application)	Gene Expression,Microbial Detection
PCR Method	qPCR
Multiplex Capability	Single-plex
Detection Method	SYBR
Includes	dUTP, UNG/UDG
Thermostability	8 hr., Assembled Reaction
Polymerase	Taq DNA Polymerase
Format	Tube
Product Type	Real Time PCR SYBR Master Mix
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio 3, QuantStudio 5, QuantStudio 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Flex, QuantStudio 7 Pro, QuantStudio 12K Flex, Bio-Rad CFX Connect, Bio-Rad CFX384 Touch, Bio-Rad CFX96 Touch, BioRad CFX96, BioRad iCycler iQ, BioRad iQ5, BioRad MyiQ, ViiA 7 System, StepOne System, MJ Chromo4, MJ Opticon, StepOnePlus System, Roche LightCycler 480, Stratagene Mx3000P, Stratagene Mx3005P, Stratagene Mx4000
Sample Type	DNA
Reaction Speed	Fast or Standard
Target Specificity	cDNA or gDNA only
Product Line	Applied Biosystems

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Applied Biosystems™ TaqMan™ Fast Universal PCR Master Mix (2X), no AmpErase™ UNG

TaqMan™ Fast Universal PCR Master Mix contains all of the components, excluding the template and primers, for superior real-time qPCR.

Pricing and Availability
Specifications

GC-Rich PCR Performance	High
Fidelity (vs. Taq)	2 X
Concentration	2X
For Use With (Application)	Gene Expression,Genotyping,miRNA analysis
PCR Method	qPCR
Detection Method	Primer-probe
Polymerase	AmpliTaq DNA Polymerase
Format	Tube
Product Type	Fast Universal PCR Master Mix
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7500 Fast System, 7900HT Fast System, StepOne™, Fast Mode, StepOnePlus™, Fast Mode
Sample Type	DNA (Genomic), cDNA
Reaction Speed	Fast
Product Line	AmpErase, TaqMan

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Applied Biosystems™ TaqMan™ Universal Master Mix II, no UNG

TaqMan Universal Master Mix II is a convenient mix of components (except primers, probes, template, and water) necessary to perform a real-time polymerase chain reaction (PCR).

Pricing and Availability
Specifications

GC-Rich PCR Performance	High
Content And Storage	Store at 2°C to 8°C in the dark. Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
Fidelity (vs. Taq)	2 X
Purity or Quality Grade	UP (Ultra Pure)
Concentration	2X
For Use With (Application)	Gene Expression,Genotyping,miRNA analysis
PCR Method	qPCR
Detection Method	Primer-probe
Polymerase	AmpliTaq Gold DNA Polymerase
Product Type	Universal Master Mix II
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7300 System, 7500 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System
Sample Type	DNA (Genomic), cDNA
Reaction Speed	Standard
Product Line	TaqMan

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Applied Biosystems™ Fast SYBR™ Green Master Mix

Designed for speed, SYBR™ Green Master Mix provides a fast, reliable, and cost-effective solution for RT-PCR application without compromising sensitivity, specificity, dynamic range, or PCR efficiency.

Pricing and Availability
Specifications

GC-Rich PCR Performance	High
Purity or Quality Grade	UP (Ultra Pure)
Concentration	2X
For Use With (Application)	Gene Expression
PCR Method	qPCR
Detection Method	SYBR
Polymerase	AmpliTaq Fast DNA Polymerase, AmpliTaq Fast UP
Product Type	Real Time PCR SYBR Master Mix
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7500 Fast System, 7900HT Fast System, StepOne™, Fast Mode, StepOnePlus™, Fast Mode
Sample Type	DNA (Genomic), cDNA
Reaction Speed	Fast
Label or Dye	SYBR Green
Product Line	SYBR

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Applied Biosystems™ TaqMan™ Fast Virus 1-Step Master Mix for qPCR

TaqMan Fast Virus 1-Step Master Mix is designed for fast, highly sensitive real-time RT-PCR, even in the presence of challenging PCR inhibitors.

Pricing and Availability
Specifications

Shipping Condition	Dry Ice
Reverse Transcriptase	M-MLV
Form	Liquid
InhibitorToleranceSimple	High
For Use With (Application)	Microbial Detection,Gene Expression,DNA Quantification,Presence/Absence
PCR Method	1-step RT-qPCR
Multiplex Capability	Up to 3-plex
Detection Method	Primer-probe
Thermostability	Not Applicable
Polymerase	AmpliTaq Fast DNA Polymerase
Format	Tube
Product Type	1-Step Master Mix
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7500 Fast System, 7500 System, 7900HT System, QuantStudio 12k Flex, QuantStudio 3, QuantStudio 5, QuantStudio 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio 7 Flex, StepOne, StepOnePlus, ViiA 7 System
Sample Type	Total RNA, Viral DNA, Viral RNA, mRNA
Reaction Speed	Fast or Standard
Target Specificity	RNA only, Both RNA and DNA
Hot Start	Built-In Hot Start
Product Line	TaqMan

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Applied Biosystems™ PowerUp™ SYBR™ Green Master Mix for qPCR

Pre-formulated, optimized, universal 2X master mix for real-time PCR workflows.

Pricing and Availability
Specifications

Shipping Condition	Wet Ice
InhibitorToleranceSimple	Medium
Concentration	2X
For Use With (Application)	Gene Expression
PCR Method	qPCR
Multiplex Capability	Single-plex
Detection Method	SYBR
Includes	dUTP, UNG/UDG
Thermostability	72 hr., Assembled Reaction
Polymerase	Dual-Lock Taq DNA Polymerase
Format	Tube
Product Type	Real Time PCR SYBR Master Mix
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7500 Fast System, 7500 System, 7900HT System, QuantStudio 12k Flex, QuantStudio 3, QuantStudio 5, QuantStudio 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio 7 Flex, StepOne, StepOnePlus, ViiA 7 System
Sample Type	DNA
Reaction Speed	Fast or Standard
Target Specificity	cDNA or gDNA only
Product Line	PowerUP, SYBR

Applied Biosystems™ TaqMan™ Fast Virus 1-Step Multiplex Master Mix for qPCR (No ROX)

TaqMan Fast Virus 1-Step Multiplex Master Mix (No ROX) is designed to enable dye flexibility for multiplexing up to four different RNA/DNA targets for high-throughput RT-qPCR.

Pricing and Availability
Specifications

Shipping Condition	Dry Ice
InhibitorToleranceSimple	High
Concentration	4X
For Use With (Application)	Microbial Detection,Gene Expression,DNA Quantification,Presence/Absence
PCR Method	1-step RT-qPCR
Multiplex Capability	Up to 4-plex
Detection Method	Primer-probe
Thermostability	Not Applicable
Passive Reference Dye	None
For Use With (Equipment)	7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 6 Pro, QuantStudio™ 7 Flex, QuantStudio™ 7 Pro, StepOne™, StepOnePlus™, ViiA™ 7 System
Sample Type	RNA, DNA
Reaction Speed	Fast or Standard
Target Specificity	RNA only, Both RNA and DNA
Hot Start	Built-In Hot Start

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Applied Biosystems™ TaqMan™ Universal Master Mix II, with UNG

TaqMan Universal Master Mix II is a convenient mix of components (except primers, probes, template, and water) necessary to perform a real-time polymerase chain reaction (PCR).

Pricing and Availability
Specifications

GC-Rich PCR Performance	High
Content And Storage	Store at 2°C to 8°C in the dark. Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
Fidelity (vs. Taq)	2 X
Purity or Quality Grade	UP (Ultra Pure)
Concentration	2X
For Use With (Application)	Gene Expression,Genotyping,miRNA analysis
PCR Method	qPCR
Detection Method	Primer-probe
Polymerase	AmpliTaq Gold DNA Polymerase
Product Type	Universal Master Mix II
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7300 System, 7500 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System
Sample Type	DNA (Genomic), cDNA
Reaction Speed	Standard
Product Line	TaqMan

Promotions Available

Applied Biosystems™ TaqMan™ Multiplex Master Mix

Applied Biosystems™ TaqMan™ Multiplex Master Mix is formulated to amplify up to 4 targets in a single reaction, delivering shorter run times (less than 40 minutes).

Pricing and Availability
Specifications

Polymerase	AmpliTaq Fast DNA Polymerase
GC-Rich PCR Performance	High
Fidelity (vs. Taq)	2 X
Product Type	Multiplex Master Mix
Sample Type	DNA
Reaction Speed	Fast
Concentration	2X
For Use With (Application)	Gene Expression,Virus Detection
PCR Method	Multiplex qPCR
Product Line	TaqMan
Detection Method	Primer-probe

Promotions Available

Applied Biosystems™ Absolute Q™ Universal DNA Digital PCR Master Mix (5X)

Absolute Q Universal DNA Digital PCR Master Mix is compatible with a broad range of applications and over 20 million Applied Biosystems predesigned TaqMan Assays, eliminating the need to design custom assays.

Pricing and Availability

Applied Biosystems™ TaqPath™ 1-Step Multiplex Master Mix (No ROX)

TaqPath 1-Step Multiplex Master Mix (No ROX) is a fast, single-tube, 4X RT-qPCR mix that provides for sensitive, reproducible detection of up to four different RNA/DNA targets in a single multiplex reaction.

Pricing and Availability
Specifications

Shipping Condition	Dry Ice
InhibitorToleranceSimple	High
For Use With (Application)	Microbial Detection,DNA Quantification,Presence/Absence
PCR Method	1-step RT-qPCR
Multiplex Capability	Up to 4-plex
Detection Method	Primer-probe
Includes	dUTP, UNG/UDG
Thermostability	Not Applicable
Sensitivity	Down to 10 copies
Format	Tube
Passive Reference Dye	None
For Use With (Equipment)	QuantStudio 6 Pro or QuantStudio 7 Pro, QuantStudio 3 or 5, QuantStudio 6/QuantStudio 7 Flex, QuantStudio 12K Flex, QuantStudio Dx, ViiA 7, 7500, 7500 Fast, 7500 Fast Dx, or use a compatible real-time PCR instrument from another supplier
Sample Type	DNA, RNA
Reaction Speed	Fast or Standard
Target Specificity	RNA only, Both RNA and DNA
Product Line	TaqPath

Applied Biosystems™ TaqPath™ ProAmp™ Multiplex Master Mix

TaqPath ProAmp Multiplex Master Mix is for higher multiplexing (up to four targets) in high-throughput genotyping and copy number variation protocols requiring uncompromising reliability and accuracy, even in the presence of inhibitors commonly found in clinical samples.

Pricing and Availability
Specifications

Shipping Condition	Wet Ice
Content And Storage	Store at 2°C to 8°C in the dark.
InhibitorToleranceSimple	Very High
For Use With (Application)	Genotyping,Pharmacogenomics
PCR Method	qPCR
Multiplex Capability	Up to 4-plex
Detection Method	Primer-probe
Includes	dUTP, UNG/UDG
Thermostability	72 hr., Assembled Reaction
Polymerase	Taq DNA Polymerase
Product Type	qPCR Master Mix
Passive Reference Dye	Mustang Purple
For Use With (Equipment)	QuantStudio 3 or 5, QuantStudio 6/QuantStudio 7 Flex, QuantStudio 12K Flex, QuantStudio Dx, ViiA 7 Real-Time PCR System ViiA 7 Dx, 7500 Fast, StepOne or StepOnePlus
Reaction Speed	Fast or Standard
Target Specificity	cDNA or gDNA only
Product Line	TaqPath

Applied Biosystems™ TaqMan™ GTXpress™ Master Mix

Combines AmpliTaq™ Fast DNA Polymerase, UP, dNTPs, Tracking Dye, and ROX™ dye in a convenient single vial format.

Pricing and Availability
Specifications

GC-Rich PCR Performance	High
Concentration	2X
For Use With (Application)	Genotyping
PCR Method	qPCR

Applied Biosystems™ TaqPath™ ProAmp™ Master Mix

TaqPath ProAmp Master Mix is for high-throughput genotyping and copy number variation protocols requiring uncompromising reliability and accuracy, even in the presence of inhibitors commonly found in clinical samples.

Pricing and Availability
Specifications

Shipping Condition	Wet Ice
Content And Storage	Store at 2°C to 8°C in the dark.
InhibitorToleranceSimple	Very High
For Use With (Application)	Genotyping
PCR Method	qPCR
Multiplex Capability	Up to 3-plex, Up to 4-plex
Detection Method	Primer-probe
Includes	dUTP, UNG/UDG
Thermostability	72 hr., Assembled Reaction
Polymerase	Taq DNA Polymerase
Product Type	qPCR Master Mix
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7500 Fast System, QuantStudio 12k Flex, QuantStudio 3, QuantStudio 5, QuantStudio 6 Flex, QuantStudio 7 Flex, ViiA 7 System, ViiA 7 Dx, QuantStudio Dx, StepOnePlus System, StepOne System
Reaction Speed	Fast or Standard
Target Specificity	cDNA or gDNA only
Product Line	TaqPath

Applied Biosystems™ TaqPath™ 1-Step Multiplex Master Mix

TaqPath 1-Step Multiplex Master Mix is a fast, single-tube, 4X RT-qPCR mix that provides for sensitive, reproducible detection of up to four different RNA/DNA targets in a single multiplex reaction.

Pricing and Availability
Specifications

Shipping Condition	Dry Ice
InhibitorToleranceSimple	High
Concentration	4X
For Use With (Application)	Gene Expression,Microbial Detection
PCR Method	1-step RT-qPCR
Multiplex Capability	Up to 4-plex
Detection Method	Primer-probe
Includes	dUTP, UNG/UDG
Thermostability	Not Applicable
Polymerase	Fast DNA Polymerase
Sensitivity	Down to 10 copies
Format	Tube
Product Type	qPCR Master Mix
Passive Reference Dye	Mustang Purple
For Use With (Equipment)	QuantStudio 6 Pro Real-Time PCR System or QuantStudio 7 Pro, QuantStudio 3 or 5, QuantStudio 6/QuantStudio 7 Flex, QuantStudio 12K Flex, QuantStudio Dx, ViiA 7, 7500 , 7500 Fast, 7500 Fast Dx
Sample Type	DNA, RNA
Reaction Speed	Fast or Standard
Target Specificity	RNA only, Both RNA and DNA
Product Line	TaqPath

Real Time PCR Master Mixes

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