Modifying Enzymes
Invitrogen™ T4 DNA Ligase Buffer
T4 DNA Ligase buffer optimizes ligation, which can be performed in 5 minutes
Invitrogen™ Terminal Deoxynucleotidyl Transferase (TdT) Buffer
Supplied with Terminal Deoxynucleotidyl Transferase (TdT)
R&D Systems™ TACS Nuclease and Buffer
Highly purified recombinant endonuclease specific for all forms of DNA
Thermo Scientific™ S1 Nuclease (100 U/μL)
Degrade single-stranded DNA and RNA with this endonuclease.
Thermo Scientific™ DNase I, RNase-free (supplied with MnCl2) (1 U/μL)
Endonuclease that nonspecifically cleaves single- and double-stranded DNA to release di-, tri-, and oligonucleotides with 5'-phosphate and 3'-OH groups.
Thermo Scientific™ FastAP Thermosensitive Alkaline Phosphatase (1 U/μL)
Catalyze the removal of 5'- and 3'-phosphate groups from DNA, RNA, nucleotides, and proteins using this thermosensitive alkaline phosphatase .
Thermo Scientific™ Terminal Deoxynucleotidyl Transferase (20 U/μL)
Terminal Deoxynucleotidyl Transferase (TdT) catalyzes the template-independent addition of deoxyribonucleotides to the 3'-OH terminus of DNA molecules.
Thermo Scientific™ Uracil-DNA Glycosylase (1 U/μL)
Uracil-DNA Glycosylase (UDG, UNG) catalyzes the hydrolysis of the N-glycosylic bond between uracil and sugar in DNA and prevents carry-over of DNA in PCR reactions.
Invitrogen™ T4 DNA Ligase (1 U/μL)
Ideal for cloning (blunt-end or cohesive-end ligation) and adding linkers or adapters to blunt-ended DNA
Macherey-Nagel™ rDNase Set
Recombinant DNase and rDNase Reaction Buffer, for RNA mini preparations with NucleoSpin RNA, NucleoSpin RNA XS, NucleoSpin miRNA, NucleoSpin RNA Blood, NucleoSpin RNA Plant, or NucleoSpin RNA/Protein kits
Thermo Scientific™ RNase H (5 U/μL)
Ribonuclease H (RNase H) is an endoribonuclease that specifically degrades the RNA strand in RNA-DNA hybrids.
Invitrogen™ SUMO Protease
A recombinant fragment of ULP1 (Ubl-specific protease 1) from Saccharomyces cerevisiae
Invitrogen™ Gateway™ BP Clonase™ II Enzyme mix
Catalyzes in vitro recombination of PCR products to generate entry clones
Thermo Scientific™ DNase I, RNase-free (1 U/μL)
Endonuclease that nonspecifically cleaves single- and double-stranded DNA to release di-, tri-, and oligonucleotides with 5'-phosphate and 3'-OH groups.