Invitrogen™ TURBO DNA-free™ Kit

Kit contains reagents for efficient, complete digestion of DNA along with removal of enzyme and divalent cations post-digestion

• Hyperactive TURBO DNase is catalytically superior enzyme compared to wild type DNase I
• Removes trace quantities of DNA that can interfere with RT-PCR
• Reagent included to completely remove DNase without phenol treatment or heating
• Method for genomic DNA removal prior to RT-PCR
• TURBO DNase is recombinant, engineered form of DNase I that is efficient than wild type DNase I in digesting away trace amounts of unwanted DNA
• TURBO DNase binds DNA substrates 6-fold more tightly than traditional DNase I, making enzyme tool of choice for clearing residual DNA that can generate a false positive signal in RT-PCR applications
• TURBO DNase now includes an enhancer that increases effectiveness by two orders of magnitude
• Efficient DNase and divalent cation removal without organic extraction or precipitation
• Conventional DNase treatment of RNA samples prior to RT-PCR typically call for inactivation of the DNase by phenol:CHCl₃ extraction or heating followed by precipitation step to concentrate the RNA
• Phenol:CHCl₃ extractions can be cumbersome and time-consuming
• Heating the sample to inactivate DNase can lead to chemical degradation of RNA by divalent cations present in DNase buffer
• TURBO DNA-free Kit circumvents problems using novel DNase Inactivation Reagent
• For removing the TURBO DNase from reaction, Inactivation Reagent also binds and removes divalent cations from TURBO DNase Reaction Buffer

PCR and Real-Time PCR, Real Time PCR (qPCR), Reverse Transcription