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Invitrogen™ Lipofectamine™ 2000 Transfection Reagent
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
Versatile transfection reagent that has been shown to effectively transfect the widest variety of adherent and suspension cell lines
Thermo Scientific™ FastDigest BamHI
Cut at G^GATCC sites with Thermo Scientific™ FastDigest™ BamHI restriction enzyme, which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer.
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | BamH I |
| Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | 10X FastDigest Buffer/FastDigest Green Buffer |
| Overhang | 5' protruding ends |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Inactivation Temperature | 80°C |
| Product Line | FastDigest |
Thermo Scientific™ EcoRI (10 U/μL)
The EcoRI restriction enzyme recognizes G^AATTC sites and cuts best at 37°C in its own unique buffer.
| Concentration | 10 U/μL |
|---|
Thermo Scientific™ HindIII (10 U/μL)
The HindIII restriction enzyme recognizes A^AGCTT sites and cuts best at 37°C in R buffer.
| Concentration | 10 U/μL |
|---|
Invitrogen™ Platinum™ SuperFi II PCR Master Mixes
Invitrogen Platinum SuperFi II PCR Master Mix (2X) is a ready-to-use mixture of Platinum SuperFi II DNA Polymerase, Platinum SuperFi II Buffer, and dNTPs for convenient PCR setup and efficient amplification.
| Shipping Condition | Dry Ice |
|---|---|
| Polymerase | Platinum SuperFi II DNA Polymerase |
| GC-Rich PCR Performance | High |
| Fidelity (vs. Taq) | >300X |
| Size (Final Product) | 20 kb or less |
| Reaction Format | SuperMix or Master Mix |
| Product Type | PCR Master Mix |
| Reaction Speed | Fast |
| Hot Start | Built-In Hot Start |
| Overhang | Blunt |
| Concentration | 2X |
| For Use With (Application) | Hot-start PCR,High-fidelity PCR |
Thermo Scientific™ DH10B Competent Cells; High Efficiency
High efficiency, chemically competent E. coli cells. The DH10B strain is suitable for cloning DNA containing methylcytosine, 5-hydroxymethylcytosine, and methyladenine, allowing both prokaryotic and eukaryotic genomic DNA to be cloned efficiently.
Thermo Scientific™ FastDigest HindIII
The FastDigest HindIII restriction enzyme recognizes A^AGCTT sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer.
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | Hind III |
| Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | 10X FastDigest Buffer/FastDigest Green Buffer |
| Overhang | 5' protruding ends |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Inactivation Temperature | 80°C |
| Product Line | FastDigest |
Invitrogen™ Qubit™ microRNA Assay Kits
Achieve accurate miRNA quantitation, even in the presence of rRNA, large mRNA, or contaminants, with the Qubit microRNA Assay Kit, which offers 100 and 500 assay formats.
| Excitation/Emission | 498/518 |
|---|---|
| Shipping Condition | Room Temperature |
| Product Type | microRNA Assay Kit |
| For Use With (Equipment) | Qubit Fluorometer |
| For Use With (Application) | microRNA Quantitation |
| Product Line | Qubit |
| Detection Method | Fluorescence |
Thermo Scientific™ FastAP Thermosensitive Alkaline Phosphatase (1 U/μL)
Catalyze the removal of 5'- and 3'-phosphate groups from DNA, RNA, nucleotides, and proteins using this thermosensitive alkaline phosphatase .
| Product Type | Alkaline Phosphatase |
|---|---|
| Enzyme | Phosphatase |
| Compatible Buffer | PCR Buffer, Enzyme Buffer |
| Concentration | 1 U/μL |
Invitrogen™ Platinum™ SuperFi II DNA Polymerase
Invitrogen Platinum SuperFi II DNA Polymerase is a proofreading DNA polymerase that combines superior fidelity with an innovative buffer, enabling universal primer annealing for the highest success in PCR.
| Shipping Condition | Dry Ice |
|---|---|
| Polymerase | Platinum SuperFi II DNA Polymerase |
| GC-Rich PCR Performance | High |
| Fidelity (vs. Taq) | >300X |
| Size (Final Product) | 20 kb or less |
| Reaction Format | Standalone |
| Reaction Speed | Fast |
| Hot Start | Built-In Hot Start |
| Overhang | Blunt |
| For Use With (Application) | Hot-start PCR,High-fidelity PCR |
Thermo Scientific™ CloneJET PCR Cloning Kit
Efficiently clone PCR products generated with any thermostable DNA polymerase using Thermo Scientific™ CloneJET™ PCR Cloning Kit, a positive selection system.
| Antibiotic Resistance Bacterial | Ampicillin (AmpR) |
|---|---|
| Format | Kit |
| Sample Type | PCR Products |
| Cloning Method | Restriction Enzyme⁄ MCS, Blunt PCR |
| For Use With (Application) | PCR Cloning |
| Vector | pJET1.2/blunt |
| Product Line | CloneJET |
Thermo Scientific™ FastDigest BglII
Cut at A^GATCT sites with FastDigest™ BglII restriction enzyme, which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer.
| Sensitive to Heat Inactivation | No |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | Bgl II |
| Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | 10X FastDigest Buffer/FastDigest Green Buffer |
| Overhang | 5' protruding ends |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Product Line | FastDigest |
Thermo Scientific™ FastDigest NcoI
The FastDigest NcoI restriction enzyme recognizes C^CATGG sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: Bsp19I).
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | Nco I |
| Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | 10X FastDigest Buffer/FastDigest Green Buffer |
| Overhang | 5' protruding ends |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Inactivation Temperature | 65°C |
| Product Line | FastDigest |
Invitrogen™ Quant-iT™ dsDNA Assay Kits, high sensitivity (HS) and broad range (BR)
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
For highly selective quantitation of double-stranded DNA over RNA, the Quant-iT dsDNA Assay Kits, high sensitivity and broad range, produce fluorescence signals that are linear in the ranges of 0.2-100 ng and 2-1000 ng of DNA, respectively.
| Shipping Condition | Room Temperature |
|---|---|
| No. of Reactions | 1000 Assays (200 μL assay volume) |
| For Use With (Equipment) | Microplate Reader |
| Quantity | 1 kit |
| Product Line | Quant-iT |
| Detection Method | Fluorescence |