PCR Reagents and Kits

Invitrogen™ MgCl2 (1M)

Ready-to-use, certified RNase-free 1 M MAGNESIUM CHLORIDE 100MLMgCl2solution, 100 ml

Invitrogen™ SuperScript™ III First-Strand Synthesis System

Optimized to synthesize first-strand cDNA from purified poly(A)+ or total RNA or RT-PCR 50 RXN FIRST STRAND SYNTHESIS SYSTEM FOR RT-PCRSuperScript(tm) III50 reactions Store at -20C

SYBR™ Fast SYBR™ Green Master Mix

Designed for speed, provides a fast, reliable and cost-effective solution for RT-PCR application without compromising sensitivity, specificity, dynamic range or PCR efficiency. 100 RXN FAST SYBR GREEN MASTER MIX MINI-PACK (1 X1mL) 100reactions Store at -20 C

Applied Biosystems™ TaqMan™ Universal PCR Master Mix

Applied Biosystems™ TaqMan™ Universal PCR Master Mix is the ideal reagent solution when you need a Master Mix for multiple 5' nuclease DNA applications. 400 RXN UNIVERSAL PCR MASTER MIX, 2 PACK (2 X

Invitrogen™ SuperScript™ IV Reverse Transcriptase

A proprietary MMLV mutant with superior robustness and reliability in RT reactions. 2000UNIT SuperScript IV Reverse Transcriptase

Thermo Scientific™ DreamTaq Green PCR Master Mix (2X)

Thermo Scientific DreamTaq Green PCR Master Mix (2X) is a ready-to-use solution containing DreamTaq DNA Polymerase, optimized DreamTaq Green buffer, MgCl2, and dNTPs. 1000RXN DreamTaq Green PCR Master Mix 20 X 1,25 mL

Thermo Scientific™ Maxima SYBR Green/ROX qPCR Master Mix (2X)

Ready-to-use solution optimized for qPCR and 2-step RT-qPCR. X4 qPCR, Maxima(R) SYBR green/ROX qPCR master

TaqMan™ TaqMan™ Gene Expression Master Mix

Precise and reliable real-time qPCR quantification over 9 logs of linear dynamic range with Applied Biosystems™ TaqMan™ Gene Expression Master Mix. 2000 RXN GENE EXPRESSION MASTER MIX, 1 BULK PACK(1 x 50mL) 2000reactions Store at 4°C

Applied Biosystems™ TaqMan™ High-Capacity RNA-to-cDNA Kit

Designed for optimum performance with TaqMan Gene Expression Master Mix, Power SYBR™ Green PCR Master Mix, and other PCR enzymes 50 RXN HIGH CAPACITY RNA-TO-CDNA KIT 50REACTIONSStore at -20 C

Thermo Scientific™ RevertAid H Minus First Strand cDNA Synthesis Kit

Perform efficient synthesis of first strand cDNA from RNA templates with this complete synthesis system. 20 RXN REVERTAID(TM) H- 1ST STRAND CDNA SYNTHESISkit, including RevertAid H- RT, RiboLock, buffer,

Invitrogen™ UltraPure™ Guanidine Isothiocyanate Solution

Strong protein denaturant for intact mRNA isolation GUANIDINE ISOTHIOCYANATE SOL.

Invitrogen™ UltraPure™ Phenol:Chloroform:Isoamyl Alcohol (25:24:1, v/v)

Nucleaic acid purfication PHENOL/CHLOROFORM/ALCOHOL

Invitrogen™ PBS Phosphate-Buffered Saline (10X) pH 7.4

Dilute to desired concentration 10X PBS BUFFER, PH 7.4 1000 MLsolution, 1000 ml

Invitrogen™ Platinum™ Taq DNA Polymerase

Convenient and reliable “hot start” thermostable DNA polymerase for PCR that provides enhanced specificity over that of Taq DNA Polymerase 120 RXN TAQ DNA POLYMERASE PLATINUM(R)ns Store at -20°C

Invitrogen™ NaCl (5M)

Ready-to-use, certified RNase-free 5M NACL 500 MLAmbion® Molecular biology grade, 5 M NaCl

Invitrogen™ Taq DNA Polymerase, Recombinant

A thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer 1000UNIT X3 Taq DNA polymerase cloned kit 500Units

Invitrogen™ SuperScript™ IV First-Strand Synthesis System

Optimized for synthesis of first-strand cDNA from purified poly(A)+ or total RNA. 200RXN SuperScript IV First-Strand Synthesis System

Thermo Scientific™ DreamTaq Green DNA Polymerase

Save time in standard PCR applications with this enhanced Taq DNA polymerase, supplied with colored buffer enabling direct gel loading. DREAMTAQ GREEN 5U/UL 200U

Thermo Scientific™ Phusion High-Fidelity DNA Polymerase

Get superior performance for cloning and other applications requiring high fidelity with one of the most accurate thermostable DNA polymerases available. 200 RXN PHUSION HIGH-FIDELITY PCR KIT, 200 U,with 5x Phusion HF buffer (3 x 1.5mL) and 5x

Invitrogen™ Sodium Acetate (3M), pH 5.5

Ready-to-use, certified RNase-free 100 ML 3 M SODIUM ACETATE, PH 5.5 100ML STORE ATroom temperature

Invitrogen™ EDTA (0.5M), pH 8.0

Ready-to-use, certified RNase-free 500 ML 0.5 M EDTA, PH 8.0 500ML STORE AT ROOMtemperature

Applied Biosystems™ MeltDoctor™ HRM Calibration Plate, Fast 96-well

These plates provide thermal and optical calibration required for accurate and reproducible discrimination between melt curves. X96 MELTDOCTOR HRM CALIBRATION 96WELL STORE AT-20 C

Invitrogen™ GlycoBlue™ Coprecipitant (15mg/mL)

Blue dye increases pellet visibility GLYCO BLUE COPRECIP 5 X 300ULAmbion® GlycoBlue consists of a blue dye

Thermo Scientific™ RevertAid™ Premium First Strand cDNA Synthesis Kit

Perform efficient synthesis of first strand cDNA from RNA templates with this complete synthesis system. 20 RXN REVERTAID(TM) 1ST STRAND CDNA SYNTHESISkit, includes RevertAid RT, RiboLock, buffer,

Applied Biosystems™ TaqMan™ Fast Advanced Master Mix

TaqMan™ Fast Advanced Master Mix has been designed to match or exceed the performance of standard master mixes, delivering shorter run times (<40 minutes). TAQMAN FAST ADVANCED MASTER MIX (1 X 50 ML)

Applied Biosystems™ AmpliTaq Gold™ DNA Polymerase with Gold Buffer and MgCl2

Chemically modified form of AmpliTaq DNA Polymerase requiring thermal activation AMPLITAQ GOLD 5X1000U

SYBR™ SYBR™ Green PCR Master Mix

Everything needed for SYBR™ Green dye–based PCR amplification and detection in a convenient, single-tube format. TF,SYBR GREEN PCR MASTER MIX,

Invitrogen™ AccuPrime™ Pfx DNA Polymerase

Provides higher fidelity than Pfu DNA polymerase 1000 RXN PFX DNA POLYMERASE ACCUPRIME(TM) 1000reactions Store at -20C

Invitrogen™ Phenol:Chloroform:IAA, 25:24:1, pH 6.6

Ready-to-use, safer than preparing crystalline phenol solutions 400 ML PHENOL:CHLOROFORM:IAA, 25:24:1, PH 6.6(raise to pH 7.9 with included buffer) 400mL

Invitrogen™ SuperScript™ III Reverse Transcriptase

Proprietary mutant of SuperScript II RT X4 RT kit Store at -20°C

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