Cell Culture
1. Sample Preparation
Preparing samples for PCR or qPCR typically requires extraction and purification of nuclear material. Depending on the sample source, this may involve mechanical manipulation via homogenization or require the use of resins or beads to help bind the nucleic acids prior to purification. Prepare your samples with the help of DNA and RNA extraction, isolation, and purification kits, plus reagents, buffers, and more.
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Featured Products
Catalog Number 11523127
Gibco™ StemPro™ EZPassage™ Disposable Stem Cell Passaging Tool
Catalog Number 4525485
Thermo Scientific™ Nunc™ Serological Pipettes
Catalog Number 161515
Fisherbrand™ SureOne™ Aerosol Barrier Pipette Tips
Resources
AcroPrep Filter Plates
- Filename
- 25524_AcroPrep_Filter_Plates.pdf
- Size
- 13 MB
- Format
- application/pdf
Benchtop Instrument Portfolio
- Filename
- 25524_Benchtop_Instrument_Portfolio.pdf
- Size
- 2 MB
- Format
- application/pdf
Cell Culture Workflow
- Filename
- 25524_Cell_Culture_Workflow.pdf
- Size
- 1 MB
- Format
- application/pdf
Essentials for Life Science
- Filename
- 25524_Essentials_for_Life_Science.pdf
- Size
- 1 MB
- Format
- application/pdf
Pall AcroPrep Cell Clarification and Sterile Filter Plates
- Filename
- 25524_Pall_AcroPrep_Cell_Clarification_and_Sterile_Filter_Plate.pdf
- Size
- 5 MB
- Format
- application/pdf
Thermo Scientific Sorvall BIOS Centrifuge Series
- Filename
- 25524_Thermo_Scientific_Sorvall_BIOS_Centrifuge_Series.pdf
- Size
- 1 MB
- Format
- application/pdf
Gibco Cell Culture Basics Handbook
- Filename
- 25524_gibco-cell-culture-basics-handbook.pdf
- Size
- 8 MB
- Format
- application/pdf
2. Cell Expansion
Your next step, cell expansion, is necessary for cell growth and will take place in a vessel containing a culture medium. There are multiple options available based on the cell line being studied. To prepare, shop products for cell expansion including media, sera, and flasks for your research. For more information on these items, download the related resources.
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Featured Products
Catalog Number 7214997
Corning™ CellBIND™ HYPERStack™ Cell Culture Vessels - New Design
Catalog Number 7058048
Cytiva HyClone™ FetalClone™ I Serum (U.S.)
Catalog Number 12359802
Cytiva Fetal Bovine Serum, US Characterized
Resources
Cleaning and Disinfection in GMP a Environment
- Filename
- Cleaning_and_Disinfection_in_GMP_Environment.pdf
- Size
- 526 KB
- Format
- application/pdf
Introducing the new, aliquot-free Gibco™ One Shot™ FBS 50mL bottle
- Filename
- Gibco-fbs-one-shot-brochure.pdf
- Size
- 9 MB
- Format
- application/pdf
Thermo Scientific Aqua-Tec Water Preservation Cell
- Filename
- Thermo_Scientific_Aqua-Tec_Water_Preservation_Cell.pdf
- Size
- 216 KB
- Format
- application/pdf
Thermo Scientific Forma Steri-Cycle Cleanroom Incubators
- Filename
- Thermo_Scientific_Forma_Steri-Cycle_Cleanroom_Incubators.pdf
- Size
- 3 MB
- Format
- application/pdf
Thermo Scientific Heracell Cleanroom Incubators
- Filename
- Thermo_Scientific_Heracell_Cleanroom_Incubators.pdf
- Size
- 3 MB
- Format
- application/pdf
Smart Note: What is a Certified Cleanroom Compatible Incubator
- Filename
- What_is_Certified_Cleanroom_Compatible_Incubators.pdf
- Size
- 759 KB
- Format
- application/pdf
3. Harvesting
Collecting and isolating cells may involve centrifugation, depth filtration, and cell detachment. Before proceeding, adherent cells should be detached from the surface of the cell culture flask or dish. Choose to harvest your cells with products from our portfolio. Find cell scrapers, lifters, and reagents, as well as resources to support your workflow.
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Featured Products
Catalog Number 3821025
Fisherbrand™ Cell Scrapers
Catalog Number 4919324
Gibco™ TrypLE™ Express Enzyme (1X), no phenol red
Catalog Number 11577692
Fisherbrand™ Cell Lifters
Resources
Thermo Scientific Herasafe 2025 Biological Safety Cabinet
- Filename
- Thermo_Scientific_Herasafe_2025_Biological_Cabinet.pdf
- Size
- 2 MB
- Format
- application/pdf
Thermo Scientific Medifuge Centrifuge
- Filename
- Thermo_Scientific_Medifuge_Centrifuge.pdf
- Size
- 947 KB
- Format
- application/pdf
4. Detection
Now that your cells are harvested, determine how you’ll read your results. There are many equipment options available to make this process more accurate and efficient. Buy counters, readers, and more to detect cells and cell colonies. Learn about microplate selection and imaging with our additional resources.
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Featured Products
Catalog Number 16832556
Invitrogen™ Countess™ 3 FL Automated Cell Counter
Catalog Number 15813915
Corning™ Cell Counter
Resources
AcroPrep Filter Plates
- Filename
- AcroPrep_Filter_Plates.pdf
- Size
- 13 MB
- Format
- application/pdf
Agilent BioTek Multimode Microplate Readers
- Filename
- Agilent_BioTek_Multimode_Microplate_Readers.pdf
- Size
- 2 MB
- Format
- application/pdf
Agilent BioTek Selection Guide
- Filename
- Agilent_BioTek_Selection_Guide.pdf
- Size
- 2 MB
- Format
- application/pdf
5. Analysis
Begin to study your cells through observation and analysis. With the right equipment, you can make this step of your workflow more effective. Choose from a variety of microscopes and microplate washers and readers for your cell culture analysis. For more information, browse our brochures and buying guides or visit our cell analysis page.
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Featured Products
Catalog Number 15918206
Invitrogen™ EVOS™ M7000 Imaging System
Catalog Number 6070001
Thermo Scientific™ Wellwash™ Versa Microplate Washer
Resources
Agilent BioTek Multimode Microplate Readers
- Filename
- Agilent_BioTek_Multimode_Microplate_Readers.pdf
- Size
- 2 MB
- Format
- application/pdf
6. Storage
There are many product options and techniques to choose from when storing your cells. Cryopreservation, for example, helps you keep cells safe long term for future research projects. Find vials, microplates, and media for cell storage. Make these products part of your workflow by learning more and placing an order.
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Featured Products
Catalog Number 4526104
Thermo Scientific™ Nunc™ Biobanking and Cell Culture Cryogenic Tubes
Catalog Number 4530722
Thermo Scientific™ Locator™ Plus Rack and Box Systems
Catalog Number 13493809
Cytiva HyClone™ Cryopreservation Media
Resources
Thermo Scientific Sample Storage Solutions
- Filename
- Thermo_Scientific_Dample_Storage_Solutions.pdf
- Size
- 5 MB
- Format
- application/pdf