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Thermo Scientific™ 5-IAF (5-iodoacetamido-fluorescein)

Product Code. 10649148
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25 mg
Unit Size:
25mg
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Product Code. 10649148 Supplier Thermo Scientific™ Supplier No. 62246

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Description

5-IAF (5-iodoacetamido-fluorescein)

Thermo Scientific 5-Iodoacetamido-Fluorescein (5-IAF) is a high-performance derivative of fluorescein dye, activated for easy and reliable labeling of antibodies, proteins and other molecules for use as fluorescent probes. 5-Iodoacetamido-Fluorescein is a sulfhydryl-reactive derivative of fluorescein that labels proteins and other molecules having free thiols (cysteine side chains)

Properties of 5-Iodoacetamido-Fluorescein:
  • Alternative names: 5-IAF, 5-iodoacetamidofluorescein
  • Chemical name: Acetamide, N-(3',6'-dihydroxy-3-oxospiro (isobenzofuran-1(3H), 9'-(9H)xanthen)-5-yl)-2-iodo
  • Molecular weight: 515.26±3
  • Excitation source: 488 nm spectral line, argon-ion laser
  • Excitation wavelength: 494 nm
  • Emission wavelength: 518 nm
  • Extinction coefficient: > 80,000 M-1cm-1
  • CAS #: 63368-54-7
  • Solubility: Soluble in DMF; aqueous buffers at pH > 6
  • Reactive groups: Iodoacetamide, reacts with sulfhydryls at pH 7.0 to 7.5

    Applications:
  • Label antibodies for use as immunofluorescent probes
  • Label oligonucleotides for hybridization probes
  • Detect proteins in gels and on Western blots
TRUSTED_SUSTAINABILITY

Specifications

Description 5-IAF (5-iodoacetamido-fluorescein), 25mg, Yellow to Orange, Powder, FITC Dye, 515.26±3 Molecular Weight, 494/518nm Excitation/Emission Wavelength, 63368-54-7 CAS Number, –20°C Storage Temperature, Used for Protein Biology, Protein Labeli
Format Powder
For Use With (Application) Protein labeling and crosslinking, protein biology
Quantity 25 mg
Product Type 5-IAF (5-iodoacetamido-fluorescein)
Molecular Weight (g/mol) 515.26±3
Purity >99% by HPLC
Content And Storage -20°C
Label or Dye FITC (Fluorescein)
Color Yellow to Orange
Emission 518
Excitation 494
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For Research Use Only. Not for use in diagnostic procedures.

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