missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Cell Analysis Products
  4. Flow Cytometry Products
  5. Flow Cytometry Compensation, Counting and Calibration
  6. Invitrogen™ AbC™ Total Antibody Compensation Bead Kit

Invitrogen™ AbC™ Total Antibody Compensation Bead Kit

Product Code. 15308666
Change view
Click to view available options
Quantity:
100 tests
25 tests
Unit Size:
100 tests
25 tests
2 product options available for selection
Product selection table with 2 available options. Use arrow keys to navigate and Enter or Space to select.
Product Code. Quantity unitSize
15308666 25 tests 25 tests
15384147 100 tests 100 tests
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 2 options available.
2 options
This item is not returnable. View return policy
Product Code. 15308666 Supplier Invitrogen™ Supplier No. A10513

Please to purchase this item. Need a web account? Register with us today!

This item is not returnable. View return policy

Description

Provides a consistent, accurate, and simple-to-use technique for the setting of flow cytometry compensation when using fluorochrome-conjugated mouse, rat, hamster, or rabbit antibodies with any laser in flow cytometry.

  • Broadest multispecies reactivity—for use with all isotypes of mouse, rat, hamster, and rabbit antibody conjugates
  • Brightest signal—most sensitive antibody-capture beads available
  • Maximum laser compatibility—for use with all lasers
  • Each kit provides polystyrene microspheres that have fluorescent antibody conjugate capture capacity (positive compensation beads) or are inert (negative compensation beads).
  • Ideal for compensation with all isotypes of mouse (IgG1, IgG2a, IgG2b, IgG3), rat (IgG1, IgG2a, IgG2b), hamster (Armenian IgG and Syrian IgG), and rabbit (monoclonal and polyclonal) immunoglobulins.Demonstrates the highest and most consistent reactivity to the different subclasses of immunoglobulin in the industry.
TRUSTED_SUSTAINABILITY

Specifications

Diameter (Metric) 6 μm
Content And Storage Contains 1 vial AbC™ Total Compensation capture beads and 1 vial of negative beads.
  • Store at 2°C to 8°C
    		•
    Format Tube
    For Use With (Equipment) Flow Cytometer
    No. of Tests 25 Tests
    Quantity 25 tests
    Detection Method Fluorescence
    Excitation Wavelength Range any lasers (UV to 633/635)
    Shipping Condition Room Temperature
    Product Line AbC
    Product Type Compensation Beads
    Show More Show Less

    Frequently Asked Questions (FAQs)

    After adding the antibody to the AbC Total Antibody Compensation beads, there does not appear to be any signal. What may have caused this?

    Here are some tips to follow:
    - Make sure that the beads were never frozen.
    - Avoid mixing by vortexing too long (no longer than 10 seconds) or sonication as this may promote denaturation of the protein on the surface of the beads.
    - Use the product within the warranty period (one year).

    Can the AbC Total Antibody Compensation Bead Kit bind Fab dimers?

    Yes, the AbC Total Antibody Compensation Bead Kit can bind Fab dimers as long as the Fab dimers are derived from either mouse, rat, hamster, or rabbit species.

    The AbC Total Antibody Compensation Bead Kit accommodates only mouse, rat, hamster, and rabbit antibodies. What can I use if my antibody is not derived from any of these species?

    As an alternative, you may covalently attach your antibody to colorless, 5 µm Aldehyde/Sulfate Latex Beads (Cat. No. A37306). The aldehyde moiety on the bead surface directly binds with primary amines on the surface of the antibodies in simple buffers such as PBS (pH 7.4). Make sure that the buffer does not contain any primary amines (i.e., do not use Tris or glycine buffers). To avoid making bead-antibody-bead-antibody multimers, add excess antibody relative to the beads.
    I am performing flow cytometry. My compensation matrix has values over 100. That's not possible is it?

    It is possible. But if you have a lot of values over 100, you probably need to look at the voltages that you are using for your data collection.
    I’ve been told that I need to compensate my data for flow cytometry analysis. What does that mean?

    By running single-color controls, it is possible to remove signal of one fluorophore that spills over into the collection channel for another fluorophore.

    What kind of controls do I need for flow cytometry?

    For compensation, you need to prepare a singly stained sample (or compensation beads) for each color parameter that you are using. In addition, we recommend that you use FMO (flow minus one) controls. These are controls in which you label cells or beads with every color in your panel, omitting one. Make one FMO control for each color. These controls are important for helping you properly set gates on your data.
    Why should I worry about compensation in flow cytometry analysis?

    In a perfect world, the fluorescence emission profile for each individual fluorophore would be a very intense, narrow peak, well separated from all other emission peaks. In reality, organic dyes and fluorescent proteins have broad emission peaks, and compensation must be employed (during or after data acquisition) to correctly assign fluorescence signal to each fluorophore. Compensation is important because it removes fluorescent signal from overlapping spectra so you know that the signal you see is only the signal from the fluorophore of interest.

    How do I make compensation controls for antibodies?

    All that you need for compensation controls is an unstained sample of your cells (or negative control beads) and samples with each of your fluorophores - one per tube. You want the single-stained controls to be as bright as you expect your brightest sample to be. Antibodies can be bound to beads instead of cells using our AbC Total Antibody Compensation Bead Kit (Cat. Nos. A10513 and A10497).

    For Research Use Only. Not for use in diagnostic procedures.

    Product Title
    Select an issue

    By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.