PeproTech Adipolean (gAcrp30) Polyclonal Antibody, PeproTech®, Invitrogen™

Description

AA Sequence of recombinant protein: MKGEPGEGAY VYRSAFSVGL ETYVTIPNMP IRFTKIFYNQ QNHYDGSTGK FHCNIPGLYY FAYHITVYMK DVKVSLFKKD KAMLFTYDQY QENNVDQASG SVLLHLEVGD QVWLQVYGEG ERNGLYADND NDSTFTGFLL YHDTN. Preparation: Produced from sera of goats immunized with highly pure Recombinant Human gAcrp30/Adipolean. Anti-Human gAcrp30/Adipolean-specific antibody was purified by affinity chromatography employing an immobilized Human gAcrp30/Adipolean matrix. Sandwich ELISA: To detect Human gAcrp30/Adipolean by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human gAcrp30/Adipolean (500-P193GBt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human gAcrp30/Adipolean. Western Blot: To detect Human gAcrp30/Adipolean by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human gAcrp30/Adipolean is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

gAcrp30 is a naturally occurring globular protein obtained by proteolytic processing of adiponectin. Adiponectin is produced and secreted exclusively by adipocytes, and is a relatively abundant plasma protein, accounting for up to 0.05% of total serum protein. Like Adiponectin, gAcrp30 is capable of decreasing hyperglycemia and reversing insulin resistance. Additionally, gAcrp30 has been shown to be an important factor in promoting fat loss by signaling muscle to absorb and burn Free-Fatty Acids (FFAs). The signaling receptors for adiponectin and gAcrp30 have recently been identified and named AdipoR1 and AdipoR2. AdipoR2 is predominantly expressed in the liver.