Learn More
Invitrogen™ MEGAshortscript™ T7 Transcription Kit
Description
The MEGAshortscript T7 Kit is designed for high yields of in vitro-transcribed RNA products, in the 20 to 500 nucleotide range. Using conventional methods, transcription of small templates often results in low mass yields of RNA. Short transcripts require many more transcription initiation events to synthesize a given mass of product. Since the initiation step is the rate-limiting step in transcription reactions, the yield of product from short templates is often low. The MEGAshortscript T7 Kit has been designed to overcome the drawbacks involved in conventional in vitro transcription to maximize the yield of short RNA transcripts.
- Efficiently transcribes small templates (<300 bases) with consistently high yields
- Designed for use with plasmid, synthetic oligonucleotide, or PCR product templates
Specifications
Specifications
| Content And Storage | T7 Enzyme Mix, 10X Reaction Buffer, ATP Solution, CTP Solution, GTP Solution, UTP Solution, pTRI-RNA 18S, TURBO DNase, Ammonium Acetate Stop Solution, and Gel Loading Buffer II are all stored at –20°C. Nuclease-free Water may be stored at any temperature. |
| Final Product Type | Synthetic mRNA, mRNA |
| Format | Kit |
| Sample Type | mRNA, Synthetic mRNA |
| For Use With (Application) | In Vitro Transcription, synthetic mRNA, mRNA, transfection, LNPs, GENEart, linearized DNA plasmid, delivery, PCR products |
| No. of Reactions | 25 Reactions |
| Product Line | Ambion, MEGAshortscript |
| Product Type | T7 In Vitro Transcription kit |
| Quantity | 25 Reactions |
| Promoter | T7 |
| Show More |
For Research Use Only. Not for use in diagnostic procedures.
By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.