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Invitrogen™ RNaseZap™ RNase Decontamination Solution
Description
RNaseZap™ RNase Decontamination Solution is a surface decontamination solution that destroys RNases on contact. You simply spray RNaseZap™ Solution onto the surface to be decontaminated and rinse it off with RNase-free water.
Features of RNaseZap™ RNase Decontamination Solution:
- Completely removes RNase contamination from glass and plastic surfaces
- Excels at removing high levels of RNase contamination whereas similar products fail
- Proven effective at removing high concentrations of dried-on RNase A
- Ideal for cleaning work surfaces, pipettors, and equipment that must be RNase-free
Using RNaseZap™ Solution
Working with RNA requires that special measures be taken to ensure an RNase-free environment. Even trace quantities of RNase can lead to lower yields from in vitro transcription reactions, degradation during RNA purification protocols, and variable results in RPAs and Northerns. RNaseZap™ contains three ingredients active against RNase and has proven to be extremely effective at removing RNase contamination from glassware, plastic surfaces, countertops, and pipettors. It has also been shown to be effective at eliminating RNase contamination from microfuge tubes without inhibiting subsequent enzymatic reactions. Supplied in one 250 mL bottle.
RNase contamination in microfuge tubes
While concern about RNase contamination of frequently handled labware is common and prudent, researchers often do not worry about RNase contamination in commercially prepared products such as microfuge tubes. However, in a survey of six standard 1.5 mL microfuge tubes in which three were pre-rinsed with RNaseZap™ and water and three were left untreated, all of the untreated tubes had at least marginal levels of RNase contamination, whereas none of the tubes pre-rinsed with RNaseZap™ exhibited contamination. Additionally, in testing commercially available RNase-free microfuge tubes, approximately 10% had some RNase contamination (data not shown). This may well account for the erratic results that researchers periodically experience when performing RNA-based assays.
Order Info
Shipping Condition: Room temperature
Specifications
Specifications
| Product Type | RNase |
| Content And Storage | Store at room temperature. |
| Shipping Condition | Room Temperature |
| Quantity | 250 mL |
| Reagent Type | RNase Control Reagent |
| Product Line | Ambion, RNaseZap |
Frequently Asked Questions (FAQs)
The formulation for RNaseZAP RNase Decontamination Solution (Cat. Nos. AM9782, AM9784, AM9780) is proprietary. The pH is 7.0-7.4.
Detection of somatic variants with the Oncomine Myeloid Research Assay GX has been verified down to 5% allele frequency.
Here is a list of kits that are compatible with Oncomine Myeloid Research Assay GX:
Nucleic acid isolation - RNA samples
- MagMAX mirVana Total RNA Isolation Kit, Cat. No. A27828
- PureLink Total RNA Blood Kit, Cat. No. K156001
- RNaseZap RNase Decontamination Solution, Cat. No. AM9780, AM9782, AM9784
Nucleic acid isolation - DNA samples
- MagMAX DNA Multi-Sample Ultra 2.0 Kit, Cat. No. A36570
- PureLink Genomic DNA Mini Kit, Cat. No. K1820-01
The recommended minimum sample input is 27.75 ng of DNA (1.11 ng/µL minimum concentration) and 14.25 ng of RNA (0.95 ng/µL minimum concentration).
The recommended controls are:
DNA Controls:
- Seraseq® Myeloid Mutation DNA Mix, Cat. No. 0710-0408 (SeraCare®)
- Myeloid DNA Reference Standard, Cat. No. HD829 (Horizon™)
- AcroMetrix™ Oncology Hotspot Control, Cat. No. 969056
RNA Controls:
- Universal Human Reference RNA, Cat. No. 740000 (Agilent™)
- Seraseq® Myeloid Fusion RNA Mix, Cat. No. 0710-0407 (SeraCare®)
For Research Use Only. Not for use in diagnostic procedures.
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