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Ampicillin
Beskrivning
Ampicillin is used as asemi-synthetic antibiotic which has antibacterial activity structurally related to penicillin. It is widely used selection reagent for transformed cells expressing β-lactamase. It is also used to prevent and treat a number of bacterial infections. It may also be used to prevent group B streptococcal infection in newborns.
This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.
General Description
- Ampicillin is a broad-spectrum, semi-synthetic, β-lactam penicillin with bactericidal activity. It acts against several gram-positive and -negative infections
- This compound binds to and inactivates penicillin-binding proteins (PBP) located on the inner membrane of the bacterial cell wall. This inactivation interferes with the cross-linkage of peptidoglycan chains essential for bacterial cell wall strength and rigidity. Consequently, there is an interruption in the bacterial cell wall synthesis which results in the weakening of the bacterial cell wall causing cell lysis
Application
- In laboratory experiments, it is commonly used as a selector reagent for transformed cells expressing β-lactamase. The ampicillin-resistant gene (ampR) catalyzes the hydrolysis of the β-lactam ring of ampicillin and naturally detoxifies the drug
- Ampicillin is commonly used as a selection marker for E. coli and other bacteria during plasmid DNA isolation, protein expression, and gene cloning
Specifikationer
Specifikationer
| Type | Ampicillin |
| Physical Form | Powder |
| Melting Point | 208°C (decomposition) |
| Color | White |
| Quantity | 5 g |
| Beilstein | 1090925 |
| Merck Index | 14,586 |
| Solubility Information | In 1 M NH4OH,50mg/ml |
| Formula Weight | 349.4 |
| Chemical Name or Material | Ampicillin |
Frequently Asked Questions (FAQs)
We recommend the following the instructions to prepare LB-ampicillin:
- Dissolve the following in 500 mL dH2O:
- 5g tryptone
- 2.5g yeast extract
- 5.0g NaCl
- 7.5g agar OR
- 20g pre-mixed LB agar powder
- Boil the solution on a hot plate while stirring for 1-2 minutes.
- Autoclave the solution for 20 minutes and let cool to 50-60°C.
- Add 25 mg ampicillin and swirl or stir on a hot plate to mix.
- Pour approximately 10 mL of molten LB agar into each plate.
- Allow plates to solidify for approximately 20 minutes.
Ampicillin, (Cat. No. J60977), is an RUO (Research Use Only) chemical. It is not intended for animal or human diagnostic or therapeutic use.
Säkerhet och hantering
- Ampicillin
- Danger
- Serious eye damage/eye irritation Category 2
- Respiratory sensitiser Category 1
- Skin corrosion/irritation Category 2
- Skin sensitiser Category 1
- Specific target organ toxicity Category 3
- H315-Causes skin irritation.
- H317-May cause an allergic skin reaction.
- H319-Causes serious eye irritation.
- H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
- H335-May cause respiratory irritation.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P302+P352-IF ON SKIN: Wash with plenty of water/soap.
- P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
- P312-Call a POISON CENTER/doctor/if you feel unwell.
- P332+P313-If skin irritation occurs: Get medical advice/attention.
- P333+P313-If skin irritation or rash occurs: Get medical advice/attention.
- P337+P313-If eye irritation persists: Get medical advice/attention.
- MIXTURE LIST-Contains : Ampicillin
RUO – Research Use Only
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