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Applied Biosystems™ TaqMan™ Sample-to-SNP™ Kit (20mL Sample prep, 50mL PCR)
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Includes
Reagents sufficient for 400 sample preparation reactions at 50μL/ reaction and 20,000 PCR reactions at 5μL for each reaction.
The TaqMan™ Sample-to-SNP™ Kit combines DNA extraction and TaqMan™ chemistry-based genotyping in a streamlined protocol to provide results from any sample in less than one hour.
- DNA Extract All Reagents kits provide PCR-ready DNA from a wide variety of sample types ranging from blood to buccal swabs to plant tissues in 5 min.
- The TaqMan™ GTXpress™ Master Mix provides robust PCR amplification of extracted DNA in less than 50 min.
- Compatible with both Fast and Standard mode thermal cycling conditions
- Pre-mixed components stored at 2° to 8°C significantly reduce assay setup time
- Validated with all types of Applied Biosystems™ TaqMan™ Genotyping Assays
- Extended PCR cycling for poorly amplified or sample-limited reactions
Order Info
Guaranteed minimum shelf life is 60 days.
Specifications
Specifications
| Content And Storage | Contains Lysis Reagents (2° to 8°C): 20mL each Lysis and DNA Stabilization Solution; Master Mix (15° to 25°C): 50mL 2X master mix with AmpliTaq™ Fast DNA Polymerase, UP, dNTPs, Tracking dye and ROX™ Dye. |
| Detection Method | Primer-probe |
| Polymerase | AmpliTaq DNA Polymerase |
| Reaction Speed | Fast or Standard |
| For Use With (Equipment) | 7300 System, 7500 Fast System, 7500 System, 7900HT Fast System, GeneAmp™ 9700, StepOne™, Fast Mode, StepOne™, Standard Mode, Veriti™ Thermal Cycler |
| Passive Reference Dye | ROX (Pre-mixed) |
| Quantity | 1 Kit |
| Target | SNPs (Known), SNPs (Unknown or Numerous) |
| For Use With (Application) | Genotyping |
| Sample Type | Buccal Sample, Cell Cultures, Hair with Follicle, Leaf Punch or Needle, Seed Chip, Tail Biopsy (Mouse), Tail Biopsy (Rat), Tissue (FFPE), Whole Blood |
For Research Use Only. Not for use in diagnostic procedures.
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