Invitrogen™ ARC Polyclonal Antibody

Description

PA1-4514 detects splice variants Nop30/ARC of the nucleolar protein Nop30 in human samples. PA1-4514 has been used successfully in Western blot procedures. By Western blot this antibody detects a ~25 kDa protein representing recombinant Nop30. The PA1-4514 immunogen corresponds to amino acid residues 125-141 of the 25 kDa human Nop30/ARC protein.

ARC (also known as NOL3 - Nucleolar protein 3) has two isoforms. Isoform 1 is involved in RNA splicing. Isoform 2 functions as an apoptosis repressor that blocks multiple modes of cell death. ARC inhibits extrinsic apoptotic pathways two different ways. The first is by interacting with FAS and FADD upon FAS activation blocking death-inducing signaling complex (DISC) assembly. The second way is by interacting with CASP8 in a mitochondria localization-and phosphorylation-dependent manner, which in turn limits the amount of soluble CASP8 avaliable for DISC-mediated activation. ARC also inhibits intrinsic apoptotic pathways in response to a wide range of stresses through interactions with BAX. This results in BAX inactivation and prevents mitochondrial dysfunction and release of pro-apoptotic factors. Mutations affecting the gene can results in myoclonus, familiam cortical.