Invitrogen™ Asialo GM1 Polyclonal Antibody, Alexa Fluor™ 488, eBioscience™

Description

Description: This rabbit polyclonal antibody reacts with the asialo ganglio-N-tetraosylceramide (asialo-GM1), a glycolipid on human, mouse, rat, bovine and hamster cells. Glycolipids such as this sialyated version are defined by the specific transferases that modify the lipid. Glycolipids are targets of immune cell response. Auto-antibodies have also been identified. Additionally these glycolipids can act as co-receptors for pathogens. Asialo-GM1 is present on both NK cells and a subset of monocyte/macrophages from the mouse spleen. Expression is also found on rat and bovine brain, fetal liver and on fetal immature thymocytes. Expression has also been shown to increase with tumor progression. The antibody recognizes asialo-GM1 specifically and not other glycolipids, including GM1 and asialo-GM2. This antibody has been reported to deplete NK cells in mice and hamsters in vivo and exhibits in vitro effects in a variety of species. Applications Reported: This polyclonal antibody has been reported for use in flow cytometric analysis. Applications Tested: This polyclonal antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 μg per test. Because this is a polyclonal antibody, there may be differences in staining brightness from lot to lot. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range...

Asialo ganglio-N-tetraosylceramide (asialo-GM1) is a glycolipid on human, mouse, rat, bovine and hamster cells. Glycolipids such as this sialyated version are defined by the specific transferases that modify the lipid. Glycolipids are targets of immune cell response. Auto-antibodies have also been identified. Additionally these glycolipids can act as co-receptors for pathogens. Asialo-GM1 is present on both NK cells and a subset of monocyte/macrophages from the mouse spleen. Expression is also found on rat and bovine brain, fetal liver and on fetal immature thymocytes. Expression has also been shown to increase with tumor progression.