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Invitrogen™ Attune™ NxT Flow Cytometer, blue/red/violet6
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Product Code. 16468710 Supplier Invitrogen™ Supplier No. A29003

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Description

Designed and developed to remove common barriers associated with flow cytometry, the evolutionary capabilities of the Attune NxT Flow Cytometer offer adaptable optical configuration options so you can get the most out of your multicolor analysis.

The 2020 Attune NxT Flow Cytometer has expanded functionalities to drive your research forward. Designed and developed to remove common barriers associated with flow cytometry, the evolutionary capabilities of the Attune NxT Flow Cytometer offer adaptable optical configuration options so you can get the most out of your multicolor analysis.

  • New CytKick and CytKick Max autosampler—more efficiency for high-throughput assays
  • New 21 CFR part 11 compliant software for regulated laboratories on Windows 10 operating system
  • Improved workflow for run protocol and instrument settings management

Fast flow

The innovative combination of acoustic focusing technology coupled with traditional hydrodynamic focusing results in acquisition speeds up to 10X faster than other flow cytometers. This technology reduces the time needed to obtain results.

  • True 35,000 event/sec (+10% coincidence rates)
  • Maximum electronic speed of 65,000 events/sec
  • Analyze small or limited samples faster than ever
  • Efficiently run traditionally long assays

The Attune NxT Flow Cytometer is ideal for immunophenotyping and signaling studies, cell cycle analysis, detection of rare events, stem cell analysis, cancer and apoptosis studies, microbiological assays, and more.

Fit for your lab today and in the future

The instrument's modular design provides the flexibility to meet future capability needs with a variety of upgrade options. Purchase what you need now and easily upgrade later. The small footprint of the instrument requires minimal bench space, even allowing use in a cell culture hood.

  • Flexibility—1 to 4 lasers, 14 colors, and 16 parameters
  • Field upgradeable—choose to add additional lasers when you decide
  • Customizable band pass filters for expanded fluorochrome choices

Tune into your data

The Attune NxT Flow Cytometer easily runs most samples including cardiomyocytes. The system is designed with a clog-resistant syringe-driven system to push the sample with a force of up to 75 psi.

  • Flexibility in sample concentrations and sample types
  • Elimination of time-consuming concentration steps with No-Wash, No-Lyse Filter Kit
  • Analysis of dilute samples, including CSF, stem cells, mouse bone marrow, mouse blood, and thin needle aspirates
  • Acquisition of a high volume of samples in minimal time
TRUSTED_SUSTAINABILITY

Specifications

Content And Storage • Attune NxT Flow Cytometer
• Minitower Windows 10 64-bit computer with Attune Cytometric Software
• Power cord with adapters
• USB cable
• Attune Shutdown Solution
• Attune Wash Solution
• Attune Focusing Fluid
• Attune NxT Flow Cell Cleaning Solution
• Attune Performance Tracking Beads
• Quick reference card
Format Tube(s), 384-well plate, 96-well plate
Laser Features Blue Excitation Laser (488 nm), Red Excitation Laser (638 nm), Violet Excitation Laser (405 nm)
Product Line Attune
Shipping Condition Room Temperature
Quantity 1 each

Frequently Asked Questions (FAQs)

My Attune NxT Flow Cytometer performance test keeps failing. What steps can I take to minimize the number of failed performance tests?

To minimize the number of failed performance tests on your Attune NxT Flow Cytometer, we suggest the following:

•   Make sure to carry out daily, weekly, and monthly maintenance procedures (pages 8-14 of the Maintenance and Troubleshooting Guide, linked below).
•   Always run 3 startup cycles and 2 Rinse procedures (pages 46-47 of the Maintenance and Troubleshooting Guide linked below).
•   If the performance test fails, carry out the following priming sequence: run 3 startup cycles, 2 de-bubble cycles, and 5 rinse procedures, followed by running the SIP sanitize function.

Please review the Attune Flow Cytometry Maintenance and Troubleshooting Guide for more information about performing routine maintenance procedures and troubleshooting.

Can I use the Attune Nxt Flow Cytometer to detect bacteria?

Yes. The Attune Nxt Flow Cytometer can detect down to 0.2 µm and using a special configuration, down to 0.1 µm. Optimize your settings using Flow Cytometry Sub-micron Particle Size Reference Kit, Cat. No. F13839.
Of all the Attune NxT filters you offer, I don't see one that matches our application. Can I use a filter other than what you offer?

Yes. You would need to purchase Attune NxT Custom Filter Holder Kit, Cat. No. A27784 to make your own custom emission or dichroic filters in order to customize the optical configuration of the instrument. Each kit contains sufficient parts to make 2 emission filters and 2 dichroic filters. The kit includes instructions for attaching the filter to the filter blade, but does not include the glass filter. Other accessories to optimize for special applications include Cat No. 100083194 Attune NxT Small Particle Side-Scatter Filter, Cat. No. 100083194 and Attune NxT Blocker Bar Conversion Kit, Cat No. A35966.

With the Attune Nxt Flow Cytometer, if I wish to only start with a single laser, can I expand to more lasers and channels?

Yes. There are 6 lasers to choose from (blue, green, yellow, red, violet, and violet 6). You can start with one laser and at any time upgrade the same instrument with up to 4 lasers to utilize a maximum of 14 detection channels. Using the Violet 6 laser along with the blue, red, and yellow lasers, one can detect up to 16 channels.

What makes the Attune Nxt Flow Cytometer better than standard flow cytometers?

Because of the acoustic-assisted hydrodynamic technology, the Attune NxT Flow Cytometer uses only 1/10th the amount of fluids, and is 10X faster and less sensitive to clogging compared to standard flow cytometers. You can set the flow rate from 12.5-1,000 µL/min and collect up to 65,000 events/sec using from 20 µL to 4 mL of sample volume. These unique properties allow for rare event analysis, as well standard flow analysis.
How often should I change filters for the Attune NxT Acoustic Focusing Cytometer?

Filters are recommended for replacement once every three months, unless performing small particle detection when once-a-month replacement is recommended.
I'd like to use the Flow Cytometry Panel Builder but will be purchasing antibodies from a different vendor than Thermo Fisher Scientific. Is this possible?

Step 2 allows for antibodies you have already purchased or are from a vendor outside Thermo Fisher Scientific. If you are undecided about which format you want for something, you can add the antigen name in the "Antibodies you need" section and then choose a placeholder fluorochrome in the following step. Both will help you keep track of channels that are reserved for reagents that you do not plan to buy from Thermo Fisher Scientific.
Can I export the spreadsheet from the Flow Cytometry Panel Builder for later use?

There are both PDF (printable) and CSV (spreadsheet) exports available in the summary step for you to take away for reference and ordering purposes.
In the Flow Cytometry Panel Builder tool, I can't find my flow cytometer in the dropdown. Can I build one?

Yes, you can define a new cytometer using the "Enter your cytometer manually" link below the cytometer dropdown menu.
I would like to use the Flow Cytometry Panel Builder but my flow cytometer does not use a standard configuration. How can I make changes to one in the dropdown?

Select a cytometer from the dropdown menu and then select the "Edit cytometer settings" link to modify the configuration as needed.
In the Flow Cytometry Panel Builder tool, what do the colorful dots indicate in the "Fluorochromes" step?

Each dot indicates a different fluorochrome that Thermo Fisher Scientific has available in each channel of the cytometer you have chosen.

How do I share my panel generated using the Flow Cytometry Panel Builder with my lab manager?

You may export the spreadsheet found on step 5 and share it.
In the Flow Cytometry Panel Builder, my marker isn't showing up under step 2 of antigen, but I see it in the catalog. How can I remedy this?

Select your target species first, and then type your antigen. A drop-down list will appear. Select the antigen from the list and click on "Confirm selections." If you have an antibody that is not on the list, in the "Antibodies you already have" section, you can enter a name and select "use this name anyway."

In the Flow Cytometry Panel Builder tool, can I go back a step without losing the work I did?

Yes, the previous work is saved. If you plan to leave the Panel Builder and come back later, be sure to select "Save" in the lower right corner to save your panel.

For Research Use Only. Not for use in diagnostic procedures.

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